Letter | Published:

Determination of Chlorogenic Acid

Nature volume 180, pages 12871288 (07 December 1957) | Download Citation

Subjects

Abstract

SLOTTA AND NEISSER1 found that o-dihydroxy-phenolic compounds such as chlorogenic acid and caffeic acid could be oxidized quantitatively with an alkaline hypoiodate solution, and used this method for the determination of these acids in coffee. They found that for every mole of chlorogenic or caffeic acid, 10 gm.-atoms of iodine were required for complete oxidation and that the oxidation involved the aromatic ring only without affecting the side chain, This method has recently been used by Wilkinson. Phillips and Bacot2 for the determination of chlorogenic acid in flue-cured tobacco. In their method the polyphenols were precipitated from an aqueous extract of tobacco with neutral lead acetate solution. The precipitate of lead salts was then washed with water to remove sugars, suspended in water and decomposed with hydrogen sulphide. Caffeic acid was removed by extraction with ether and chlorogenic acid estimated in the resulting solution by the above method1. This solution may, however, contain other substances which possess o-dihydroxyphenolic groups, such as rutin3,4, isoquercitrin5,6 and quercetin5. Wilkinson et al.2 state without any proof that rutin did not interfere to any appreciable extent. Since these authors used their results for drawing conclusions about the quality of flue-cured tobacco, it was decided to investigate the action of alkaline hypoiodate on pure polyphenols which contained o-dihydroxyphenolic groups.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , and , Ber., 71, 1616 (1938).

  2. 2.

    , , and , J. Assoc. Official Agric. Chem., 37, 1004 (1954).

  3. 3.

    , and , Naturwiss., 23, 800 (1935).

  4. 4.

    , and , U.S. Dept. Agric. Bur. Agric. Indust. chem. Processed Cir., AIC-52 (1944).

  5. 5.

    , , and , Arch. Biochem., 25, 74 (1950).

  6. 6.

    , Report of Symposium on Vegetable Tannins held at Cambridge, 75 (1956).

  7. 7.

    , Biochem. J., 53, 337 (1953).

  8. 8.

    , Anal. Chem., 24, 1444 (1952).

  9. 9.

    , and , Ber. deut. Botan. Ges., 66, 76 (1953).

Download references

Author information

Affiliations

  1. Research Department, The Imperial Tobacco Company, Ltd., Raleigh Road, Bristol, 3.

    • A. S. WEAVING

Authors

  1. Search for A. S. WEAVING in:

About this article

Publication history

Published

DOI

https://doi.org/10.1038/1801287a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.