Letter | Published:

Isolation of Intact Liver Cells

Nature volume 180, pages 12831284 (07 December 1957) | Download Citation

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Abstract

LIVER cells have been isolated by various methods, such as by forcing the tissue through stainless steel sieves1, by tryptic digestion2 and by incubation in acid solutions3. Anderson4 obtained a high yield of cells by perfusion of liver with calcium-free solutions containing chelating agents (such as ethylenediamine tetraacetate (versene) or citrate) and subsequent dispersion.

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References

  1. 1.

    , Exp. Cell Res., 7, 568 (1954).

  2. 2.

    , and , J. Exp. Med., 99, 167 (1954).

  3. 3.

    , and , Nature, 177, 997 (1956).

  4. 4.

    , Science, 117, 627 (1953).

  5. 5.

    , and , Aust. J. Sci., 19, 72 (1956).

  6. 6.

    , and , Biochem. J., 63, 647 (1956).

  7. 7.

    , J. Biochem. Biophys. Cyt., 2, 65 (1956).

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Author information

Author notes

    • R. K. MORTON

    Present address: Department of Agricultural Chemistry, The Waite Institute, University of Adelaide.

Affiliations

  1. Department of Biochemistry, University of Melbourne.

    • MARJORIE V. BRANSTER
    •  & R. K. MORTON

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https://doi.org/10.1038/1801283a0

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