Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Isolation of Intact Liver Cells

Nature volume 180, pages 1283–1284 (1957)Cite this article

Abstract

LIVER cells have been isolated by various methods, such as by forcing the tissue through stainless steel sieves1, by tryptic digestion2 and by incubation in acid solutions3. Anderson4 obtained a high yield of cells by perfusion of liver with calcium-free solutions containing chelating agents (such as ethylenediamine tetraacetate (versene) or citrate) and subsequent dispersion.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Kaltenbach, J. P., Exp. Cell Res., 7, 568 (1954).

    Article  CAS  Google Scholar 

  2. Dulbecco, R., and Vogt, M., J. Exp. Med., 99, 167 (1954).

    Article  CAS  Google Scholar 

  3. Longmuir, I. S., and ap Rees, W., Nature, 177, 997 (1956).

    Article  ADS  CAS  Google Scholar 

  4. Anderson, N. G., Science, 117, 627 (1953).

    Article  ADS  CAS  Google Scholar 

  5. Branster, M. V., and Morton, R. K., Aust. J. Sci., 19, 72 (1956).

    Google Scholar 

  6. Kamphausen, H., and Morton, R. K., Biochem. J., 63, 647 (1956).

    Article  CAS  Google Scholar 

  7. Novikoff, A. B., J. Biochem. Biophys. Cyt., 2, 65 (1956).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. R. K. MORTON

    Present address: Department of Agricultural Chemistry, The Waite Institute, University of Adelaide,

Authors and Affiliations

  1. Department of Biochemistry, University of Melbourne,

    MARJORIE V. BRANSTER & R. K. MORTON

Authors
  1. MARJORIE V. BRANSTER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. K. MORTON
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

BRANSTER, M., MORTON, R. Isolation of Intact Liver Cells. Nature 180, 1283–1284 (1957). https://doi.org/10.1038/1801283a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/1801283a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing