Abstract
THE deoxyribonucleic acid content of the whole small intestine of mice and rats has been used as a measure of cell population in order to follow intestinal damage after X-irradiation, and has been found to be more reliable than simply measuring the intestinal weight. The method of Schmidt and Thannhauser1 was used for separating the deoxyribonucleic acid and its concentration measured by Allen's method2 of phosphorus determination and by ultra-violet spectrophotometry. At least three animals from different litters were used for each time interval after each radiation dose, and a control animal from each litter. The mean total deoxyribonucleic acid per small intestine from 43 control mice was found to be 0.72 mgm. The individual values were dependent on body-weight.
References
Schmidt, G., and Thannhauser, S. J., J. Biol. Chem., 161, 83 (1945).
Allen, R. J. L., Biochem. J., 34, 858 (1940).
Bond, V. P., and Cronkite, E. P., Ann. Rev. Physiol., 19, 299 (1957).
Salerno, P. R., and Friedell, H. L., Western Reserve University Atomic Energy Medical Research Report NYO 4924 (1955).
Nerurkar, M. K., Baxi, A. J., Ranadive, N. S., Narurkar, M. V., and Sahasrabudhe, M. B., Nature, 180, 193 (1957).
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MOLE, R., TEMPLE, D. Intestinal Damage by Radiation and its Chemical Modification. Nature 180, 1278 (1957). https://doi.org/10.1038/1801278a0
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DOI: https://doi.org/10.1038/1801278a0
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