Two-Dimensional Agar Electrophoresis of Serum Mucoproteins


THE agar electrophoresis technique developed in this laboratory1,2 has been adapted to two-stage and two-dimensional procedure for demonstrating the micro-heterogeneity of the main protein components of human serum3. Recently, Markham4 reported a method of two-dimensional paper electrophoresis for the separation of mucoproteins. The method is elaborate, and the separations achieved are not satisfactory, diffused zones being obtained as a result of trailing due to adsorption of the proteins on paper at the acid PH. The present communication gives a brief description of a simple method of two-dimensional agar electrophoresis, by which the mucoproteins can be separated into well-defined zones.

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  1. 1

    Giri, K. V., Naturwiss., 43, 232 (1956).

  2. 2

    Giri, K. V., J. Ind. Inst. Sci., 38, 190 (1956).

  3. 3

    Giri, K. V., Naturwiss., 43, 448 (1956).

  4. 4

    Markham, R. L., Nature, 177, 125 (1956).

  5. 5

    Giri, K. V., Naturwiss., 43, 226 (1956).

  6. 6

    Giri, K. V., J. Lab. and Clin. Med., 48, 775 (1956).

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GIRI, K. Two-Dimensional Agar Electrophoresis of Serum Mucoproteins. Nature 179, 632 (1957).

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