Isolation of Nucleic Acids from Micrococcus lysodeikticus


WE have found a simple method for the preparation of a mixture of ribonucleic acid and deoxyribonucleic acid and the preparation of purified deoxyribonucleic acid in a highly polymerized state from cells of M. lysodeikticus. In arriving at the techniques, we have taken advantage of the fact that above pH 8.0 the activity of the ribonuclease system in M. lysodeikticus is negligible. Moreover, in the absence of orthophosphate, polynucleotide phosphorylase (polyase) is inactive toward ribonucleic acid. Therefore, for maximum yields of ribonucleic acid the lysis of the cells was carried out at pH 8.5. To destroy the ribonucleic acid selectively orthophosphate was added1. This communication presents some of the preliminary results.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Beers, jun., R. F., Nature, 178, 595 (1956).

  2. 2

    Beers, jun., R. F., Science, 122, 1016 (1955).

  3. 3

    Beers, jun., R. F., Nature, 177, 790 (1956).

  4. 4

    Vendrely, R., Polmade, C., and Vendrely, C., Nature, 178, 1044 (1956).

  5. 5

    Frisch-Niggermeyer, W., Enzymologia, 16, 72 (1953).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

BEERS, R. Isolation of Nucleic Acids from Micrococcus lysodeikticus . Nature 179, 630–631 (1957) doi:10.1038/179630b0

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.