Histological and Histochemical Changes in Gamma-irradiated Lima Beans, Phaseolus lunatus

Abstract

CLARK'S Bush variety of lima bean was grown on the farm of Farmington Experiment Station, Farmington, Utah; pods were harvested when beans were in the prime canning stage and were placed and sealed under vacuum in No. 10 tin cans. Each can was labelled, indicating the dose of gamma-rays to be given. The cans thus prepared for irradiation were transported under refrigeration (40° F.) to the National Reactor Testing Station, Arco, Idaho. The radiation used in the experiment was from a source of active spent fuel rods. Irradiation was by exposing the spent fuel rods from the reactor in a canal containing approximately 18 ft. of water. The rods were placed under columnar racks, and the cans containing lima beans were placed on the racks. The rate of irradiation was 1 × 106 rep/hr. at 78° F. The beans received the following doses of radiation: 0 × 106 (control), 1 × 106, 2 ×106, 4 × 106, 8 × 106, 10 × 106, 20 × 106, 40 × 106, 60 × 106, 70 × 106, 80 × 106 and 100 × 106 rep.

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References

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    Roberts, E. A., Plant Physiol., 31, xi (1956).

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SALUNKHE, D. Histological and Histochemical Changes in Gamma-irradiated Lima Beans, Phaseolus lunatus. Nature 179, 585–586 (1957). https://doi.org/10.1038/179585b0

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