IN a recent survey of the organic acids of fruits, shikimic acid has heen found in considerable amount in each of the varieties of gooseberry examined. Aqueous extracts of unripe fruits of the varieties Leveller, Careless and Whinham's Industry, after precipitation of pectin with 60 per cent acetone, were investigated by paper chromatography using the following three solvents: (1) benzyl alcohol–iso-propyl alcohol – tert.-butyl alcohol – water – formic acid (3:1:1:1:2 per cent)1, (2) n-propyl alcohol – cone. ammonia (70:30)2, (3) phenol – water – formic acid (3:1:1 per cent)1. In addition to malic and citric acid spots, a third spot (A) was observed with RF values in solvents (2) and (3), 0.17 and 0.58 respectively, identical with those of shikimic acid; citric acid and shikimic acid do not separate in solvent (1). Spot A separated from quinic and dihydroshikimic acids in solvent (3)3, RF values 0.58, 0.50 and 0.66 respectively, and rapidly gave a greenish-yellow colour at room temperature on spraying with sodium metaperiodate followed by sodium nitroprusside and piperazine4. This reagent is so far only known to give a reaction with quinic, shikimic and dihydroshikimic acids3. It has now been found that shikimic acid reacts at room temperature within a few minutes, while the other acids react more slowly. This observation is an additional aid to identification.
Subscribe to Journal
Get full journal access for 1 year
only $3.83 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Stark, J. B., Goodban, A. E., and Owens, H. S., Anal. Chem., 23, 413 (1951).
Isherwood, F. A., and Hanes, C. S., Biochem. J., 55, 824 (1953).
Phillips, J. D., Pollard, A., and Whiting, G. C., J. Sci. Food Agric., 7, 31 (1956).
Cartwright, R. A., and Roberts, E. A. H., Chem. and Indust., 230 (1955).
Hulme, A. C., Nature, 178, 991 (1956).
Davis, B. D., “A Symposium on Amino Acid Metabolism”, edited by McElroy, W. D., and Glass, H. B., 802 (The Johns Hopkins Press, Baltimore, 1955).
Boldt, A., Pharm. Zentr., 78, 157 (1937), through Br. Abstr. A.2, 194 (1937).
Hulme, A. C., and Richardson, A., J. Sci. Food Agric., 5, 221 (1954).
Richardson, A., and Hulme, A. C., Nature, 175, 43 (1955).
Hathway, D. E., Biochem. J., 63, 380 (1956).
About this article
Shikimic acid: review of its analytical, isolation, and purification techniques from plant and microbial sources
Journal of Chemical Biology (2012)
Journal of the Institute of Brewing (1973)
Journal of the Institute of Brewing (1972)
Analytical Biochemistry (1972)