Abstract
A NEW approach to the structure and functions of the cell surface of certain bacteria was revealed when Weibull1 showed that, in the presence of sucrose, lysozyme dissolves the cell-wall, leaving the protoplast essentially intact. Various attempts have since been made to isolate protoplasts from bacteria normally insensitive to lysozyme2 and also from yeast. Thus Nečas3 showed that spontaneously autolysing yeast gave rise to a small extent to structures which, superficially at least, resembled protoplasts.
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References
Weibull, C., J. Bact., 66, 688 (1953).
Lederberg, J., Proc. U.S. Nat. Acad. Sci., 42, 574 (1956). Zinder, N. D., and Arndt, W. F., ibid., 42, 586 (1956). Mitchell, P., and Moyle, J., J. Gen. Microbiol., 16, 184 (1957). Repaske, R., Biochim. Biophys. Acta, 22, 189 (1956)
Nečas, O., Nature, 177, 898 (1956).
Giaja, J., C.R. Soc. Biol., Paris, 86, 708 (1922).
Eddy, A. A., and Rudin, A. D., Proc. Soc. Gen. Microbiol., London (April 1957).
Wickerham, L. J., Tech. Bull. U.S. Dept. Agric., No. 1029 (1951).
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EDDY, A., WILLIAMSON, D. A Method of isolating Protoplasts from Yeast. Nature 179, 1252–1253 (1957). https://doi.org/10.1038/1791252a0
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DOI: https://doi.org/10.1038/1791252a0
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