Synthesis Of 4-Methylumbelliferone β-D-Glucuronide, a Substrate for the Fluorimetric Assay of β-Glucuronidase

Abstract

AT the present time there is considerable demand for a suitable synthetic substrate for the routine assay of β-glucuronidase. The most common assay method employs phenolphthalein glucuronide isolated from rabbit urine1. However, the preparation of 4-methylumbelliferone glucuronide from rabbit urine and its use in the fluorimetric assay of β-glucuronidase have been recently described2. This substrate promises certain advantages over phenolphthalein glucuronide, and it is easy to synthesize by the method previously employed for (−)-menthol3 and phenol4 β-D-glucuronides.

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