A Culture Technique for Venturia Spp. and a Turbidimetric Method for the Estimation of Comparative Sporulation


GROWTH and sporulation of Venturia inaequalis and V. pirina have been extensively studied in artificial culture. Miller1 obtained objective measures of sporulation and also concluded that inoculum concentrations greater than 200,000 conidia per ml. had little effect on the final yield of conidia. Montgomery and Moore2 grew cultures of V. inaequalis on sterilized apple twigs from which the bark had been removed. They obtained rapid growth and sporulation, and could remove most of the conidia easily without disintegration of the substrate, by shaking the cultures with water. In the following methods the advantages of a firm substrate have been coupled with the use of media of known or constant composition for studies of comparative sporulation under various nutritional conditions, and for the provision of routine supplies of conidia for fungicide bioassay.

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  1. 1

    Miller, H. J., Phytopathology, 39, 245 (1949).

  2. 2

    Montgomery, H. B. S., and Moore, M. H., J. Pomol., 15, 253 (1937).

  3. 3

    Kirkham, D. S., Nature, 173, 690 (1954).

  4. 4

    Kirkham, D. S., and Flood, A. E., Nature, [178, 422; 1956].

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