Abstract
A MAJOR problem in toxicological analysis is the separation of the poison from metabolites and body constituents that are co-extracted. This problem is especially acute in cases of poisoning by alkaloids. One technique that is of great use in solving this problem is paper chromatography1–3. The next step in the analysis is the identification of the alkaloid by such techniques as ultra-violet spectrophotometry, micro-colour tests and observations of the form of crystals obtained with various reagents. Recently, Clarke and Williams4 have described a technique whereby 0.05 ml. of a solution containing 50 µgm. of alkaloid provides sufficient material for five hundred separate micro-colour and crystal tests. This quantity of alkaloid (50 µgm.) is about the amount most suited for initial purification by paper chromatography. We have, however, found difficulty in eluting the alkaloid from the chromatogram and concentrating the eluate into 0.05 ml. of solution without loss of alkaloid. A simple and highly efficient apparatus for accomplishing this operation is shown in Fig. 1.
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References
Mannering, Dixon, Carroll and Cope, J. Lab. Clin. Med., 44, 292 (1954).
Munier, Macheboeuf and Cherrier, Bull. Soc. Chim. Biol., 34, 204 (1952).
Curry, A. S., and Powell, H., Nature, 173, 1143 (1954).
Clarke and Williams, J. Pharm. Pharmacol., 7, 255 (1955).
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TRYHORN, F., CURRY, A. An Apparatus for eluting and concentrating Chromatogram Spots. Nature 178, 1180 (1956). https://doi.org/10.1038/1781180a0
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DOI: https://doi.org/10.1038/1781180a0
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