Abstract
THE presence of modified cytochrome c in the widely used Keilin and Hartree preparation was first reported by Margoliash1. Separation was effected by chromatography at pH 7.0 on a column of the resin ‘Amberlite IRC-50’ Modified material was present to the extent of 10–25 per cent and was characterized by having a lowered enzymatic activity in the cytochrome oxidase (heart muscle) and succinic oxidase (kidney) systems but an increased rate of ascorbic acid oxidation. Visual spectroscopy, however, did not show any difference between this and other cytochrome c fractions. Evidence was presented that the modification was caused by the action of trichloracetic acid on cytochrome c during the extraction procedure.
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References
Margoliash, E., Biochem. J., 56, 535 (1954).
Henderson, R. W., and Rawlinson, W. A., Biochem. J., 62, 21 (1956).
Ball, E. G., Symposium on Respiratory Enzymes, 21 (Univ. Wisconsin Press, Madison, 1942).
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HENDERSON, R., RAWLINSON, W. Oxidation-Reduction Potential of Modified Cytochrome c . Nature 177, 1180–1181 (1956). https://doi.org/10.1038/1771180b0
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DOI: https://doi.org/10.1038/1771180b0
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