FILTER-PAPER ionophoresis is now being widely used for separations of mixtures of neutral sugars, which are converted into charged molecules by the formation of borate complexes1. This excellent method provides a far more rapid separation of isomeric components which possess appropriate structural differences than does filter-paper chromatography ; and at the same time, deductions can be made about the probable structure of an unknown sugar from its rate of migration. It does, however, suffer from a disadvantage when compared with filter-paper chromatography, inasmuch as it does not give any indication of the molecular size of an unknown oligosaccharide. We now report a different method of filter-paper ionophoresis which readily yields this information; it is to be regarded as complementary to the borate process. When both methods are used in conjunction, much information about a new product can be gained very rapidly.
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BARKER, S., BOURNE, E., GRANT, P. et al. Ionophoresis of Oligosaccharides as N-Benzylglycosylammonium Ions. Nature 177, 1125 (1956). https://doi.org/10.1038/1771125a0
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