Isolation of Microsporum gypseum and of Keratinomyces ajelloi from Australian Soil

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SIXTY-SIX samples of soil collected from various parts of New South Wales were examined for the presence of keratinophilic fungi. Following the technique of Vanbreuseghem1, Petri dishes half-filled with moistened soil were baited with autoclaved human hair. At varying intervals, ranging from three to seven weeks, some hairs were found to be overgrown by fungi; these were examined microscopically and transferred to Sabouraud's agar containing ‘Actidione’ (cycloheximide), penicillin and streptomycin2.

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  1. 1

    Vanbreuseghem, R., Ann. Soc. Beige Méd. Trop., 32, 173 (1952).

  2. 2

    Ajello, L., J. Invest. Dermat., 21, 157 (1953).

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