Respiratory Chain Phosphorylation in Extracts of Azotobacter vinelandii


RESPIRATORY chain phosphorylation has been demonstrated in crude extracts of a few bacterial species1–4, and in one case the extract was separated into three components which were all necessary for phosphorylation coupled with the oxidation of reduced diphosphopyridine nucleotide2. However, the elements supporting the system of enzymes responsible for oxidative phosphorylation in bacterial cells is still unknown. The present communication describes some preliminary experiments in which a crude water-extract from Azotobacter vinelandii was fractionated by high-speed centrifugation into particles and ‘soluble’ fractions. This organism was chosen because it is strictly aerobic (cf. ref. 2) and has a very high respiratory activity.

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TISSIÉRES, A., SLATER, E. Respiratory Chain Phosphorylation in Extracts of Azotobacter vinelandii . Nature 176, 736–737 (1955).

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