Letter | Published:

Brucella abortus Strain 19 cultivated in Aerated Liquid Medium

Naturevolume 175page999 (1955) | Download Citation

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Abstract

AERATED liquid culture methods1 have been evolved to produce Brucella vaccine for cattle at greatly reduced cost. The shake-flask technique was employed. Rectangular flasks of four-litre capacity, containing 450 ml. liquid culture, were agitated on a shaking machine at 64 excursions per min. of 12 cm. each. The medium2 consisted of 3 per cent peptone, 3 per cent glucose, 1 per cent ‘Marmite’ (yeast extract), 0.16 per cent disodium hydrogen phosphate (mol. wt. 141.98), 0.03 per cent ‘Antifoam’ (polyoxethylene derivative of ricinoleic acid supplied by I.C.I., Ltd.) in tap-water; pH 6.4.

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References

  1. 1

    Glassman, H. N., and Elberg, G. S., J. Bact., 52, 423 (1946).

  2. 2

    McCullough, W. C., Mills, R. C., Herbst, T. E., Roessler, W. G., and Brewer, C. R., J. Bact., 53, 5 (1947). Van Der Schaaf, A., Tijdschr. Diergen., 73, 598 (1948).

  3. 3

    Van Drimmelen, G. C., J. S. Afr. Vet. Med. Assoc., 23, 149 (1952).

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  1. Section of Bacteriology, Onderstepoort, Transvaal

    • G. C. VAN DRIMMELEN

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https://doi.org/10.1038/175999a0

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