Abstract
AERATED liquid culture methods1 have been evolved to produce Brucella vaccine for cattle at greatly reduced cost. The shake-flask technique was employed. Rectangular flasks of four-litre capacity, containing 450 ml. liquid culture, were agitated on a shaking machine at 64 excursions per min. of 12 cm. each. The medium2 consisted of 3 per cent peptone, 3 per cent glucose, 1 per cent ‘Marmite’ (yeast extract), 0.16 per cent disodium hydrogen phosphate (mol. wt. 141.98), 0.03 per cent ‘Antifoam’ (polyoxethylene derivative of ricinoleic acid supplied by I.C.I., Ltd.) in tap-water; pH 6.4.
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Van Drimmelen, G. C., J. S. Afr. Vet. Med. Assoc., 23, 149 (1952).
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VAN DRIMMELEN, G. Brucella abortus Strain 19 cultivated in Aerated Liquid Medium. Nature 175, 999 (1955). https://doi.org/10.1038/175999a0
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DOI: https://doi.org/10.1038/175999a0
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