Abstract
DURING a study undertaken to evaluate the ultrafiltration method for concentration of gonadotrophic hormones from urine1, we investigated the role of adsorption in this procedure. It is well known that adsorption participates in ultrafiltration2. In his article Gorbman suggests the possible importance of adsorption in the method of isolation of gonadotrophic hormones. Grabar (personal communication, 1950) reported adsorption of urinary gonadotrophic hormones when urine was allowed to drip through a nitrocellulose column. No quantitative results, however, seem to have been reported in the literature so far.
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References
Gorbman, A., Endocrin., 37, 177 (1947).
Bechhold, H., “Die Kolloide in Biologie u. Medizin”, 111 (Dresden, 1919). Elford, W. J., Proc. Roy. Soc., B, 112, 384 (1933). Grabar, P., “Études Physicochimiques Appliquées à la Biologie”, 90 (Paris, 1943).
Van Gilse, H. A., thesis (Leyden, 1953). Van Gilse, H. A., Nass, C., and Kassenaar, A. A. H. (to be published).
Klinefelter, H. F., Albright, F., and Griswold, G. C., J. Clin. Endocrin., 3, 529 (1943).
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VAN GILSE, H. Adsorption as a Major Phenomenon in the Concentration of Gonadotrophins by Ultrafiltration. Nature 175, 686–687 (1955). https://doi.org/10.1038/175686a0
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DOI: https://doi.org/10.1038/175686a0
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