Letter | Published:

Simultaneous Evaluation of Circulating Eosinophils, Polymorphonuclears and Lymphocytes by Phase-contrast Microscopy

Naturevolume 175page1128 (1955) | Download Citation

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Abstract

WHILE counting blood eosinophils, it may be very useful to determine the corresponding numbers of the other white cells in the same preparation. This can be effected in a simple and reliable way, by using phase-contrast microscopy and a modified Zollikofer's solution1, composed of 1 ml. yellow eosin (2 per cent aqueous solution, kept in a refrigerator), 3 ml. formalin (40 per cent, neutralized with calcium carbonate) and distilled water to make up 100 ml.

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References

  1. 1

    Zollikofer, R., Z. Mikrosk. u. mikr. Tech., 17, 313 (1900).

  2. 2

    Kendall, M. G., “Rank Correlation Methods”, 37 (1948).

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Affiliations

  1. Research Laboratory, Department of Gynæcology and Obstetrics, University of Ghent

    • P. J. CARPENTIER

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https://doi.org/10.1038/1751128a0

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