Abstract
THE two most commonly used methods for detecting deoxyribose compounds are the diphenylamine reaction1 and the cysteine–sulphuric acid method2,3, both originally discovered by Dische. Overend and Stacey4 have made the most recent survey of the usefulness of these methods. Both methods suffer from the same fault, that neither measures pyrimidine-bound deoxyribose quantitatively. Brody5 has used the cysteine–sulphuric acid method under conditions of precise temperature control for the quantitative determination of deoxyribose nucleic acid. It was not determined whether or not the pyrimidine-bound deoxyribose compounds were as chromogenic as the purine-bound sugars. The observations presented here show that the method can be employed for a variety of compounds containing deoxyribose.
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References
Dische, Z., Mikrochemie, 8, 4 (1930).
Dische, Z., Proc. Soc. Exp. Biol. Med., 55, 217 (1944).
Stumpf, P. K., J. Biol. Chem., 169, 367 (1944). Manson, L. A., and Lampen, J. O., J. Biol. Chem., 191, 87 (1951).
Overend, W. G., and Stacey, M., “Adv. in Carb. Chem.”, 8, 45 (1953).
Brody, A., Acta Chem. Scand., 7, 502 (1953).
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MANSON, L. Colour Reaction for Deoxyribose Compounds. Nature 174, 967 (1954). https://doi.org/10.1038/174967a0
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DOI: https://doi.org/10.1038/174967a0
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