Abstract
IN a previous communication1, we reported that when cyanocobalamin was added to certain crude liver extracts, assays carried out by means of a mutant of Escherichia coli gave much lower results than would have been expected from the amount of vitamin B12added, although Lactobacillus leichmannii gave fairly satisfactory recoveries. It was suggested that such extracts contained a substance that interfered with the utilization of vitamin B12 by E. coli. We have since obtained additional results on the nature of this interfering factor, and the publication of two recent papers2,3 on the presence of bound forms of vitamin B12 in human serum and in sow's milk respectively prompts us to put our preliminary results on record.
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ROBINSON, F., FITZGERALD, M., FEHR, K. et al. Vitamin B12 in Crude Liver Extracts. Nature 174, 558–559 (1954). https://doi.org/10.1038/174558a0
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DOI: https://doi.org/10.1038/174558a0
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