Abstract
GOODCHILD1 has recently described attempts to culture in vitro the tissues of Rhodnius prolixus, and suggested two possibilities that might enable the successful establishment of cultures. The two suggestions are (1) to replace glass surfaces by mica, or cover them with a film of wax, and (2) to enrich the culture medium with an extract of R. prolixus embryos. Similar suggestions were included in a series of experiments that I have recently performed. Cultures capable of growth and of yielding subcultures have not so far been established, and it appears that the problems of insect tissue culture are more complex than has hitherto been appreciated.
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References
Goodchild, A. J. P., Nature, 173, 504 (1954).
Trager, W., J. Exp. Med., 61, 501 (1935).
Buck, J. B., Chapter 6 in Roeder's “Insect Physiology” (John Wiley, N.Y., 1953).
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GRACE, T. Culture of Insect Tissues. Nature 174, 187–188 (1954). https://doi.org/10.1038/174187b0
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DOI: https://doi.org/10.1038/174187b0
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