Abstract
IN isolating mycorrhizal fungi present in root tissues, the external surfaces must be sterilized without damaging the hyphæ within. Existing techniques involving chemical sterilization or removal of the epidermis under aseptic conditions were found to be unsatisfactory in Calluna vulgaris and Vaccinium myrtillus, as they involved loss of hyphæ in the epidermis where the hyphæ were concentrated. Accordingly, a centrifuging technique was devised which obviated these difficulties.
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HOPKINS, J. A Centrifuging Method for Root Sterilization. Nature 174, 1016 (1954). https://doi.org/10.1038/1741016a0
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DOI: https://doi.org/10.1038/1741016a0
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