Abstract
THE standard procedure for detecting phosphorus-32 in deoxyribonucleic acid by autoradiography is to hydrolyse the smears or sections in N HCl at 60° C. for 6–10 minutes. The acid hydrolysis removes RNA and most of the non-DNA phosphates.
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References
Lajtha, L. G., J. Clin. Path., 5, 67 (1952); Exp. Cell Res., 3, 696 (1952).
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LAJTHA, L. Detection of Adenine Carbon-14 in Deoxyribonucleic Acid by Autoradiography. Nature 173, 587–588 (1954). https://doi.org/10.1038/173587a0
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DOI: https://doi.org/10.1038/173587a0
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