Salt Interference in Sugar Chromatography of Urine


DURING a recent investigation of lactosuria in burns1 following cutaneous absorption of the lactose from penicillin-lactose powder, it was found that ascending chromatography of the urine yielded spots of considerably lower RF values than lactose itself. 0.02 ml. amounts of urine were applied; and the solvent consisted of n-butanol 5.7 parts, ethanol 1.57 parts, water 2.73 parts, and contained 1 per cent ammonia solution. The substance obtained from these spots, however, when isolated and identified by standard chemical procedures, proved to be lactose.

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  1. 1

    Baar, S., and Bull, J. P., Lancet, i, 978 (1952).

  2. 2

    Kennedy, E. P., and Barker, H. A., Anal. Chem., 23, 1033 (1951).

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BAAR, S., BULL, J. Salt Interference in Sugar Chromatography of Urine. Nature 172, 414–415 (1953).

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