Abstract
RECENT studies have demonstrated the positive image of the Golgi substance in epithelial cells of the mouse epididymis with the phase-contrast microscope1. In situ, the Golgi substance was found to possess characteristic optical properties with phase-contrast and dark-field illumination, and to be completely refractory to staining with methylene blue and neutral red. Examination of cells of the head of the epididymis of the rat and the mouse immediately after or during crushing clearly indicated that the Golgi substance retained its general form and its specific optical properties upon release from its intra-cellular environment. Experiments to determine the most suitable medium for the maintenance of the Golgi substance extracellularly indicated that the presence of 0.34 M sodium chloride was essential and that the addition of sucrose in concentrations ranging from 0.25 to 1.25 M to the saline medium had no appreciable effect on the morphology, optical properties or stability of the Golgi substance.
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References
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SCHNEIDER, W., DALTON, A., KUFF, E. et al. Isolation and Biochemical Function of the Golgi Substance. Nature 172, 161–162 (1953). https://doi.org/10.1038/172161b0
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DOI: https://doi.org/10.1038/172161b0
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