Abstract
DURING a study1 of the hydrolysis of native hog thyroglobulin labelled with iodine-131, by a purified protease isolated from hog thyroid glands, fractions eluted from paper chromatograms of the enzyme fission products were treated with 2N sodium hydroxide at 105° for 16 hr. Control aliquots were allowed to stand for the same time at 0°. On application of n-butanol extracts of the heated and control samples to paper and development of the chromatograms with n-butanol – n-pentanol – ammonia2 as solvent, unexpected radioactive spots of high RF (at 0.52 and 0.68) were observed with the heated samples. Since the original eluates were found to contain free mono- and di-iodo-tyrosine and thyroxine, the action of alkali on purified samples of the iodo-amino-acids was examined.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
Purchase on Springer Link
Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Gordon, A. W., McQuillan, M., Stanley, P. G., and Trikojus, V. M., Proc. Oxford Isotope Conference, 1951.
Hird, F. J. R., and Trikojus, V. M., Aust. J. Sci., 10, 185 (1948).
Lemmon, R. M., Tarpey, W., and Scott, K. G., J. Amer. Chem. Soc., 72, 758 (1950).
Roche J., Michel, R., and Lafon, M., Biochim. et Biophys. Acta, 1, 453 (1947).
Friedberg, W., and Reineke, E. P., Fed. Proc., 11, 50 (1952).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
STANLEY, P. Artefacts produced from 3-Iodo-Tyrosine and 3 : 5-Di-iodo-Tyrosine by Heating with Alkali. Nature 171, 933–934 (1953). https://doi.org/10.1038/171933b0
Issue Date:
DOI: https://doi.org/10.1038/171933b0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.