Paper Chromatography of Œstrogens


THE Burton, Zaffaroni and Keutman1 method for the paper chromatographic analysis of the adrenal cortical steroids has been found to be suitable for the non-steroid œstrogens of the hexœstrol series (hexœstrol, stilbœstrol, dienœstrol, trienœstrol2) and ethinyl œstradiol. It can also be used for the natural œstrogens (private communication from H. Grundy). Development is carried out using the toluene – propylene glycol system, and the detecting reagent, a mixture of 1 per cent ferric chloride and 1 per cent potassium ferricyanide as used by Barton, Evans and Gardner3, gave a blue spot with less than 2 µgm./sq. cm. of the run synthetic œstrogen. In addition, stilbœstrol, dienœstrol, trienœstrol but not hexœstrol gave a strong absorption at 2300 A., and dienœstrol a blue and trienœstrol a green fluorescence at 3600 A.

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  1. 1

    Burton, R., Zaffaroni, A., and Keutman, E., J. Biol. Chem., 188, 763 (1951).

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  2. 2

    Dodds, E. C., Huang, R. L., Lawson, W., and Robinson, Sir Robert, Proc. Roy. Soc., [B, 140, 470 (1953)].

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  3. 3

    Barton, G. M., Evans, R. S., and Gardner, J. A. F., Nature, 170, 249 (1952).

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  4. 4

    Allan, H., Dickens, F., and Dodds, E. C., J. Physiol., 68, 348 (1930).

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JELINEK, P. Paper Chromatography of Œstrogens. Nature 171, 750–751 (1953).

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