Abstract
THE separate isolation and estimation of the three œstrogens in tissue at present entails the use of a large amount of material, with a separation by partition in solvents which is by no means perfect. The resulting product is very impure and has to be assayed biologically rather than chemically. Various Chromatographic and other techniques have been tried to find a method of purification and separation1–3, all of which for various reasons were found to be unsatisfactory for tissue extracts.
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MITCHELL, F. Chromatographic Isolation and Estimation of the Natural Œstrogens from Tissue. Nature 170, 621–622 (1952). https://doi.org/10.1038/170621b0
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DOI: https://doi.org/10.1038/170621b0
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