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Engraftment

Differential role for very late antigen-5 in mobilization and homing of hematopoietic stem cells

Bone Marrow Transplantation volume 38, pages 789–797 (2006)Cite this article

Abstract

The role of very late antigen-5 (VLA-5) in homing and mobilization of hematopoietic stem cells from normal bone marrow (NBM) and bone marrow (MBM) and peripheral blood (MPB) from mobilized mice was investigated. We found a decreased number of VLA-5-expressing cells in the lineage-negative fraction of MPB. However, virtually all stem/progenitor cells were present in the VLA-5+ fraction and hence mobilization of hematopoietic stem cell subsets does not coincide with a downregulation of VLA-5. Stem/progenitor cells from MPB and MBM demonstrated enhanced stromal-derived factor-alpha-induced migration. This enhanced migration correlates with an improved hematopoietic reconstitution potential, with the migrated MPB cells showing the fastest reconstitution. Interestingly, homing of MPB, MBM and NBM stem/progenitor cells in bone marrow and spleen did not differ and is therefore not responsible for the differences in hematopoietic reconstitution. The observed increase in VLA-5+ cells in the recipients after transplantation can most probably be attributed to selective homing of VLA-5+ cells instead of an upregulation of VLA-5. Treatment with an antibody to VLA-5 partially inhibited bone marrow homing of progenitor cells, whereas homing in the spleen was hardly affected. These data indicate a differential role for VLA-5 in the movement of stem cells from and toward bone marrow.

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  1. Department of Cell Biology, Section of Stem Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

    P K Wierenga, E Weersing, B Dontje, G de Haan & R van Os

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Correspondence to P K Wierenga.

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Wierenga, P., Weersing, E., Dontje, B. et al. Differential role for very late antigen-5 in mobilization and homing of hematopoietic stem cells. Bone Marrow Transplant 38, 789–797 (2006). https://doi.org/10.1038/sj.bmt.1705534

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