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Original Articles

Kinetics of dendritic cell chimerism and T cell chimerism in allogeneic hematopoietic stem cell recipients

Bone Marrow Transplantation volume 37, pages 57–64 (2006)Cite this article

Abstract

Dendritic cells (DC) as potent antigen-presenting cells (APC) and T cells as effector cells play an essential role in the pathophysiology of both graft-versus-host (GvH) and graft-versus-leukemia (GvL) reactions after transplantation. Therefore, we determined the kinetics of DC and T-cell chimerism establishment after allogeneic hematopoietic cell transplantation (AHCT) in a group of 144 patients, using fluorescence-activated cell sorting (FACS) or magnetic cell sorting (MACS) followed by FISH or STR-PCR analysis for chimerism evaluation. In all, three cell lines investigated (CD3+ T cells, CD11c+ DC1 and CD123+ DC2), we found a rapid and consistent establishment of complete donor chimerism (CDC) in over 70% of all patients during the first 6 weeks after AHCT. The rate of patients with CDC increased significantly over time within the first year after transplantation. A related donor (P=0.004) as well as an underlying lymphatic leukemia (P=0.03) were found to be significantly associated with development of MC in T cells. No significant correlation between DC or T cell chimerism and GvHD or relapse was detected. Our results thus demonstrate a fast and stable CDC in DC1, DC2 and T cells after AHCT that continuously increases over time in nearly all patients.

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Acknowledgements

This work is part of the doctoral thesis of Moritz Hamann.

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Authors and Affiliations

  1. Department of Internal Medicine III, Klinikum Grosshadern, Ludwig-Maximilians-University of Munich, Munich, Germany

    S Boeck, M Hamann, V Pihusch, T Heller, R Pihusch & H-J Kolb

  2. Institute for Clinical Chemistry, Klinikum Grosshadern, Ludwig-Maximilians-University of Munich, Munich, Germany

    H Diem

  3. Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Munich, Germany

    B Rolf

  4. Clinical Cooperative Group ‘Hematopoietic Cell Transplantation’, GSF – National Research Center for Environment and Health, Neuherberg, Germany

    H-J Kolb

  5. Abteilung fuer Haematologie und Internistische Onkologie, Klinikum der Universitaet Regensburg, Regensburg, Germany

    M Pihusch

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  1. S Boeck
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Correspondence to S Boeck.

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Boeck, S., Hamann, M., Pihusch, V. et al. Kinetics of dendritic cell chimerism and T cell chimerism in allogeneic hematopoietic stem cell recipients. Bone Marrow Transplant 37, 57–64 (2006). https://doi.org/10.1038/sj.bmt.1705217

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