Abstract
The aim of this study was to detect donor-derived hepatocytes and gastrointestinal epithelial cells in recipients of sex-mismatched allogeneic hematopoietic cell transplants, and to assess the effect of tissue injury on the extent of the repopulation. A total of 29 paraffin-embedded biopsy samples were reviewed. Double labeling by immunohistochemistry and fluorescence in situ hybridization was performed. Eighty-nine percent of sex-mismatched samples with histologic evidence of injury demonstrated the presence of donor-derived hepatocytes and gastrointestinal epithelial cells (mean 2.4%). None of the hepatocytes and gastrointestinal epithelial cells in samples obtained from female recipients with female donors showed a Y chromosome signal. The proportion of donor-derived hepatocyte and gastrointestinal epithelial cells in samples with severe graft-versus-host disease was greater than that of samples with mild/moderate graft-versus-host disease (P=0.09). No relationship between the source of stem cells and the population rate was detected (P>0.05). We conclude that some recipient hepatocytes and gastrointestinal tract epithelial cells are replaced by donor-derived cells during tissue injury. The severity of tissue injury seems to influence on the extent of this repopulation.
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Acknowledgements
We thank Muhip Ozkan, MD, Ankara University Agriculture Faculty, Department of Biometry-Genetics, for his assistance with statistical analysis; Ajlan Tukun, MD, Ankara University School of Medicine, Department of Medical Genetics, for her generous help in using the image analyzer system; and Yasemin Sahin, Ankara University Biotechnology Institute, and Klara Dalva Ankara University School of Medicine, Hematology Laboratory, for their technical assistance. Ramazan Idilman and Mutlu Arat have been supported by the Turkish Academy of Sciences, in the framework of the Young Scientist Award Program (EA-TUBA-GEBIP/2001-1-1, 2004-1-1).
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Idilman, R., Kuzu, I., Erden, E. et al. Evaluation of the effect of transplant-related factors and tissue injury on donor-derived hepatocyte and gastrointestinal epithelial cell repopulation following hematopoietic cell transplantation. Bone Marrow Transplant 37, 199–206 (2006). https://doi.org/10.1038/sj.bmt.1705214
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DOI: https://doi.org/10.1038/sj.bmt.1705214