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Engraftment

Engraftment kinetics of human CD34+ cells from cord blood and mobilized peripheral blood co-transplanted into NOD/SCID mice

Bone Marrow Transplantation volume 35, pages 271–275 (2005)Cite this article

Summary:

We have reported short periods of post transplant neutropenia in human patients co-transplanted with cord blood (CB) and low numbers of haploidentical mobilized peripheral blood (MPB) CD34+ cells. To investigate the effect that the proportion of MPB to CB cells may have on engraftment kinetics, we have co-transplanted fixed numbers of human CB CD34+ cells mixed with different numbers of MPB CD34+ cells into NOD/SCID mice. We periodically quantified the proportion of human cells and the relative contribution of MPB and CB cells to the human engraftment on marrow aspirates. At the lowest MPB/CB ratios (5 : 1, 10 : 1), the contribution of CB cells predominated at all time points analyzed, and in three out of four experiments MPB cell contributions progressively decreased from day +15. At higher MPB/CB ratios, MPB cells had a more important contribution to both early and late engraftment, with the highest cell ratio resulting in only marginal CB cell engraftment. Therefore, our results showed greater potential, on a per cell basis, of human CB vs MPB cells for competitive sustained engraftment in the xenogeneic model used, which was only abrogated by the co-infusion of very high numbers of MPB cells.

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Acknowledgements

We thank D Sánchez-Mora and Y Gutiérrez (CPH), and Jesús Martínez (CIEMAT) for technical assistance. This work was conducted as part of the Eurocord III Project and with partial support of the FIS Grant PI-03/0961.

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Author notes

  1. M Ramírez

    Present address: Oncología, Hospital Niño Jesús, Madrid, Spain

Authors and Affiliations

  1. Unidad de Hematopoyesis, CIEMAT, Hospital Puerta de Hierro, Universidad Autónoma de Madrid, Madrid, Spain

    M Ramírez & J A Bueren

  2. Servicio de Hematología y Hemoterapia, Hospital Puerta de Hierro, Universidad Autónoma de Madrid, Madrid, Spain

    C Regidor & M N Fernández

  3. Hospital Clínico, Universidad de Valencia, Spain

    I Marugán & J García-Conde

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  1. M Ramírez
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  2. C Regidor
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  3. I Marugán
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  4. J García-Conde
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  5. J A Bueren
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Correspondence to M N Fernández.

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Ramírez, M., Regidor, C., Marugán, I. et al. Engraftment kinetics of human CD34+ cells from cord blood and mobilized peripheral blood co-transplanted into NOD/SCID mice. Bone Marrow Transplant 35, 271–275 (2005). https://doi.org/10.1038/sj.bmt.1704765

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