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Endothelial Colonies

In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation

Bone Marrow Transplantation volume 32, pages 1165–1173 (2003)Cite this article

Summary:

The distribution and functional characteristics of in vitro bone marrow (BM) endothelial colonies (CFU-En) were studied in 70 non-Hodgkin's lymphoma (NHL) patients in different phases of the disease to explore the association between CFU-En growth and angiogenesis, and between the number of CFU-En and the presence of hematopoietic and mesenchymal progenitor cells. The mean number of CFU-En/106 BM mononuclear cells seen in remission patients was significantly higher than that seen in newly diagnosed patients (P=0.04), and in normal subjects (P=0.008). Patients with low-grade NHL in remission displayed a higher CFU-En value compared with high-grade NHL (P=0.04). In the autograft group (40 patients), a significant reduction of CFU-En number was detected in the first 4–6 months after transplantation. In remission patients, the CFU-En number positively correlated with the incidence of BM colony-forming unit granulocyte–macrophage (CFU-GM) (P=0.013) and CFU-multilineage (CFU-GEMM) hematopoietic colonies (P=0.044). These in vitro data show that CFU-En numbers increase following standard-dose chemotherapy, thus providing a rationale for further investigating the effects of different cytostatic drugs on BM endothelial cells growth and function.

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Acknowledgements

This work is supported by a grant from CNR/MIUR (Bridge project).

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Authors and Affiliations

  1. Department of Biomedical Sciences and Advanced Therapies, Section of Hematology, University of Ferrara, Italy

    F Lanza, D Campioni, M Punturieri, S Moretti, R Spanedda & G Castoldi

  2. Onco-Hematology Unit, International Cancer Centre, Rovigo, Italy

    M Dabusti

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  1. F Lanza
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  2. D Campioni
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  3. M Punturieri
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  4. S Moretti
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  7. G Castoldi
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Correspondence to F Lanza.

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Lanza, F., Campioni, D., Punturieri, M. et al. In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation. Bone Marrow Transplant 32, 1165–1173 (2003). https://doi.org/10.1038/sj.bmt.1704279

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