Abstract
THE various methods used so far in preparing chromosomes for electron microscopy can broadly be classified into the following three categories: (1) cutting ultra-thin sections, (2) isolation of chromosomes, and (3) making squash preparations. Electron micrographs of the chromosomes of grasshoppers have been made by employing all these techniques. The material consisted of the spermatogonial cells and spermatocytes from the testes of adult male grasshoppers. The micrographs were taken by means of the new electron microscope installed in the Institute of Nuclear Physics, Calcutta, details of which have been published elsewhere1. Comparatively low magnifications were used purposely because we did not like the chromosomes to be magnified beyond recognition in our first attempt.
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References
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GUHA, A., DE, M. & RAY-CHAUDHURI, S. Electron Microscopic Study of Grasshopper Chromosomes. Nature 170, 360–361 (1952). https://doi.org/10.1038/170360a0
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DOI: https://doi.org/10.1038/170360a0
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