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Progenitor Cell Homing

Enumeration of bone marrow ‘homing’ haemopoietic stem cells from G-CSF-mobilised normal donors and influence on engraftment following allogeneic transplantation

Bone Marrow Transplantation volume 28, pages 1019–1022 (2001)Cite this article

Abstract

Expression of the chemokine receptor CXCR4 on human haemopoietic stem cells (HSC) may play a crucial role in localising these cells to the bone marrow. To evaluate whether CXCR4 expression is clinically relevant we have enumerated CXCR4-positive HSC used for allogeneic transplantation and sought any relationship with the rate of subsequent haemopoietic reconstitution. CD34-positive progenitor cells were isolated from peripheral blood stem cell (PBSC) collections from 16 normal donors. The proportion of cells co-expressing CXCR4 was enumerated and the times to recipient haemopoietic reconstitution measured. The median frequency of CD34-positive cells co-expressing CXCR4 was 41% (range 16% to 76%) and the median number of CXCR4 CD34 double-positive cells infused at transplantation was 2.5 × 106 cells/kg (range 0.8–10.3). Patients receiving >2.5 × 106 CXCR4 CD34 double-positive cells/kg demonstrated a significant shortening of time to platelet engraftment compared to the recipients of the lower cell doses (10 days vs 14.5 days, respectively, P = 0.02) with all but one of the recipients of the higher cell doses achieving platelet engraftment by day 11. Co-expression of CXCR4 on CD34-positive progenitor cells may be an important determinant of post-transplant engraftment and in our hands transplantation of a minimum of 2.5 × 106 CXCR4 CD34 double-positive cells/kg ensured rapid post-transplant platelet recovery.

Bone Marrow Transplantation (2001) 28, 1019–1022.

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Acknowledgements

This work was funded in part by the Anderson Trust and by Amgen Australia.

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  1. BMT Programme, Alfred Hospital, Melbourne, Australia

    A Spencer, J Jackson & C Baulch-Brown

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  1. A Spencer
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  2. J Jackson
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Spencer, A., Jackson, J. & Baulch-Brown, C. Enumeration of bone marrow ‘homing’ haemopoietic stem cells from G-CSF-mobilised normal donors and influence on engraftment following allogeneic transplantation. Bone Marrow Transplant 28, 1019–1022 (2001). https://doi.org/10.1038/sj.bmt.1703289

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