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T Cell Depletion

Excessive T cell depletion of peripheral blood stem cells has an adverse effect upon outcome following allogeneic stem cell transplantation

Abstract

We evaluated the outcome of two modes of T cell depletion for HLA-identical sibling stem cell transplants in 34 consecutive adult patients: group A (n = 11) received PBSC post CliniMACs immuno-magnetic enrichment of CD34+ cells and group B (n = 23) received bone marrow following in vitro incubation with CAMPATH-1M and complement. All patients received an identical conditioning regimen which consisted of in vivoCAMPATH-1H 20 mg over 5 days, thiotepa 10 mg/kg, cyclophosphamide 120 mg/kg and 14.4 Gy TBI. No additional graft-versus-host disease prophylaxis was given. The mean T cell dose administered was 0.02 ± 0.05 × 106/kg for group A and 2.8 ± 2.8 106/kg for group B (P < 0.001). With a median follow-up of 28 months overall survival was 36.4% for group A at 12 months compared to 78.3% for group B (P = 0.001). Transplant-related mortality in group A at 12 months was 63.6% as compared to 18.0% in group B (P = 0.003). Most of the procedure-related deaths in group A occurred secondary to infection. These results suggest that extensive in vitro T cell depletion of peripheral blood stem cells in combination with in vivo T cell depletion may have profound effects upon the incidence of infections following allogeneic stem cell transplantation and this may adversely effect transplant-related mortality. Bone Marrow Transplantation (2001) 28, 827–834.

Main

T cell depletion (TCD) is an effective means of reducing the incidence and severity of acute or chronic graft-versus-host disease (GVHD) following allogeneic stem cell transplantation (SCT).1,2,3,4 A number of different methods for TCD have been developed over the last two decades that include counter-flow elutriation,2 lectin agglutination,4 monoclonal antibodies directed at T cell antigens with narrow5 or broad specificity1 or depletion through positive selection of CD34+ stem cells.6,7 The clinical benefit of TCD allogeneic SCT is controversial since this approach may be compromised by increased rates of graft rejection8 and a higher rate of relapse.9 To overcome the risk of graft rejection additional approaches have been adopted that include increasing pre-transplant immunosuppression,10 ‘partial’ TCD11 or increasing the number of stem cells administered by using peripheral blood stem cells (PBSCs).12,13 The optimal T cell content of a graft that maintains a significant graft-versus-leukaemia (GVL) effect has not yet been defined. Although TCD is used widely by many different transplant centres, few studies have assessed directly how different modes of TCD affect clinical outcome.

One area of potential concern is the significant delay in immune reconstitution that occurs following TCD allogeneic SCT. This is particularly the case in adults, where the initial T cell repertoire is dependent upon peripheral expansion of mature T cells in the graft.14,15,16 Delayed recovery of CD4+, CD4+CD45RA+ and TCRγδ+ T cells with a concomitant reduction in TCR diversity are typical features of the early post-transplant period following TCD allogeneic SCT.16,17,18,19 T cell purging may thus exacerbate post-transplant immunodeficiency and be complicated by an increased incidence of opportunistic infections, particularly CMV re-activation.20

In this report, we highlight the major impact on clinical outcome of two approaches to in vitro TCD, using either positive selection of CD34+ cells from PBSC or CAMPATH 1M treatment of bone marrow, as part of a protocol that employed additional in vivo TCD. Patients who received donor PBSCs heavily depleted of T cells (5 log) by immuno-magnetic selection of CD34+ cells had a substantially higher procedure-related mortality than recipients of CAMPATH 1M-treated bone marrow. The higher number of procedure-related deaths was caused by a marked increase in the number of opportunistic infections within the TCD PBSC group. This study suggests that following HLA-identical sibling SCT in adults, extensive in vitro TCD of PBSC in combination with in vivo TCD is complicated by a profound immunodeficiency that outweighs any benefit in terms of reduction of GVHD.

Patients and methods

A total of 34 consecutive adult patients who received a TCD HLA-identical sibling donor stem cell transplant at University College Hospital London, UK between January 1997 and October 1999, were included in the analysis. During this period, the method of in vitro TCD was changed due to an insufficient supply of CAMPATH 1M. The protocols were approved by the local institutional review committees and all patients gave informed consent. Two groups of patients were defined according to the mode of T cell depletion employed: ‘group A’ consisting of 11 patients who received PBSC following CliniMACs immuno-magnetic enrichment of CD34+ cells and ‘group B’ comprising 23 who received bone marrow following in vitro incubation with CAMPATH 1M and complement.

Patient characteristics

The patient characteristics for each group are shown in Table 1. Patients were considered to be ‘standard risk’ in the case of acute myeloid leukaemia (AML) in first complete remission (CR) or chronic myeloid leukaemia (CML) in first chronic phase.21 All other diagnoses were considered high risk. There were no significant differences between the groups in terms of age, sex, performance status at the time of transplant, time to transplant or those at risk of CMV disease (Table 1). However, only one patient in group A was considered to be high risk as compared to 11 of 23 patients in group B (P = 0.05). This reflected, in part, the lack of patients with lymphoproliferative disorders in group A. Three patients in group B had received a previous SCT and none in group A.

Table 1 Comparison of groups A and B

Antibodies

CAMPATH-1H is a humanized IgG1 monoclonal antibody against the CD52 antigen.22 It was prepared from the culture supernatant of Chinese hamster ovary cell transfectants cultured in a hollow fibre fermentor. It was purified by affinity chromatography on Protein A sepharose and size exclusion chromatography on Superdex 200 and formulated in phosphate-buffered saline. The half-life of CAMPATH-1H in humans is dependent on the amount of target CD52 antigen in the patient. Based on work in progress, there is persistence of CAMPATH-1H in vivo post day 0 sufficient to cause T cell lysis by ADCC. CAMPATH-1M is a rat IgM antibody that recognises the same antigen. It was prepared from hybrid myeloma cells using stirred fermentors, purified by fractionation with ammonium sulphate and reformulated in phosphate-buffered saline.

Conditioning regimen

All patients received the same conditioning regimen which consisted of in vivo CAMPATH-1H 20 mg on days −9 to day −5, thiotepa 5 mg/kg on days −8 and −7, cyclophosphamide 60 mg/kg on days −6 and −5 and 14.4 Gy total body irradiation, with partial lung shielding, in eight fractions over 4 days.

For stem cell collection for group A patients, sibling donors received G-CSF at 10 μg/kg subcutaneously once daily on day −4 to day 0. Leukaphereses were performed on day 0 ± day +1 using conventional techniques for PBSC. TCD was performed by positive immuno-magnetic selection of CD34+ cells using a CliniMACs, (Miltenyi Biotec, Bergisch Gladbach, Germany) cell separation system.23

For group B patients, donor bone marrow was aspirated under general anaesthesia and TCD performed in vitro upon the derived buffy coats by incubation with 25 mg CAMPATH 1M at room temperature for 10 min followed by incubation with 10–30% autologous plasma at 37°C for 45 min.

For both protocols, the level of TCD was monitored by flow cytometric analysis of CD3 staining.

Supportive care

Patients were managed in reverse isolation in conventional or laminar air flow rooms. All patients received prophylaxis with cotrimoxazole or pentamadine against Pneumocystis carinii infection. Aciclovir and triazole prophylaxis were routinely used. Blood products were irradiated to 25 Gy. Red cell and platelet transfusions were given to maintain the Hb >9 g/dl and platelet count >10 × 109/l. Patients who were CMV seronegative received only blood products from CMV seronegative donors; seropositive patients received blood products from donors unscreened for CMV. Febrile neutropenic patients received intravenous piptazobactam and gentamicin as first line antibiotic therapy. Patients received G-CSF at 5 μg/kg/day from day +6 until the ANC was at least 1.0 × 109/l for 2 consecutive days. CMV seropositive patients were monitored weekly from transplantation until at least day 120 by qualitative PCR of CMV DNA from peripheral blood. Pre-emptive ganciclovir therapy (5 mg/kg twice daily intravenously or adjusted according to renal function) was given following two consecutive positive PCR results and discontinued after 2 weeks if a negative PCR was obtained.24 In the event of continued PCR positivity, foscarnet was substituted for ganciclovir and the drugs alternated every 2 weeks according to the PCR results. A single patient (UPN5) received cidofovir as initial pre-emptive therapy for PCR detection of CMV re-activation.

GVHD prophylaxis and grading

No additional GVHD prophylaxis was given. Patients who survived 100 days or longer were evaluable for chronic GVHD. Both acute GVHD and chronic GVHD were graded according to the consensus criteria.25,26

Evaluation of infective complications

An infective complication was defined as any infection occurring post day 21 which required continued or new hospital admission/referral. CMV re-activation was defined as two consecutive peripheral blood PCR positive results. CMV disease was diagnosed on the basis of an inflammatory process due to CMV confirmed by the presence of typical cytopathic and immuno-fluorescent features in histological preparations or positive detection of early antigen fluorescent foci (DEAFF) and/or CMV culture from relevant material such as washings from broncho-alveolar lavage (BAL). Pulmonary fungal infection was diagnosed either by histological confirmation or characteristic high resolution CT appearances (halo sign) plus positive cultures from a BAL. Fungal infection at other sites was identified from post-mortem histological analysis of affected organs. RSV, parainfluenza I and III or influenza B infection were defined as pulmonary signs plus direct immunofluorescence and/or culture for the relevant viruses from naso-pharyngeal aspirate or BAL. Confirmation of Legionaire's disease was made by the presence of urinary Legionella antigen on two consecutive occasions and confirmed by post mortem histological examination of lung. Adenoviral, RSV and measles infection were confirmed by histological examination of the relevant organs postmortem. Pneumonia was defined as fever, associated with signs of lung consolidation and new infiltrates identified on chest X-ray or high resolution CT.

Statistical analysis

Overall survival (OS) was measured from transplantation until death from any cause. Patients still alive at the time of the analysis were censored at the last follow-up date. Transplant-related mortality (TRM) was determined from the date of transplantation until death related to transplantation. Patients who died from other causes were censored at the time of death. OS and TRM were estimated by the Kaplan–Meier method and the significance of differences between the curves was estimated by the log rank test. Patient characteristics in the two groups were compared by Fisher's exact test or the Mann–Whitney test, whichever was appropriate.

Results

T cell depletion

Patients in group A received approximately 2 log less T cells than group B patients (Table 2). The mean T cell dose was 0.02 ± 0.05 × 106/kg for group A recipients and 2.8 ± 2.8 106/kg for group B patients (P < 0.001). Three patients in group A had ‘add-back’ of donor T cells following the transplant (Table 2). Two of these patients, UPN6 and UPN8, received 1 × 106/kg T cells at 4 and 5 months, respectively, because of progressive pulmonary infections. UPN3 was given 3 × 106/kg T cells for cytogenetic relapse of CML at 10 months. Two patients in group B received donor T cells. UPN17 received 3 × 106/kg T cells at 11 months for relapsed AML and UPN20 received 2 × 108/kg T cells for treatment of relapsed multiple myeloma.

Table 2 Patient and graft characteristics

Engraftment

The mean CD34+ cell dose per patient was 4.4 ± 1.8 × 106/kg in group A and 1.7 ± 1.0 × 106/kg in group B (P < 0.001). Neutrophil engraftment occurred more rapidly in group A, with neutrophils >0.5 × 109/l on day 12.0 ± 2.1 compared to day 17.2 ± 4.0 for group B (P < 0.001). One patient in group B failed to engraft and died on day +74. All patients in group A engrafted. No cases of secondary graft failure occurred in either group. No differences were observed between the groups in terms of transfusional independence by day 100 (four of seven evaluable patients in group A as compared to 15 of 22 evaluable patients in group B were transfusion independent).

GVHD

The incidence of acute GVHD was low in both groups (Table 3). No patients in group A developed acute GVHD of greater than grade I as compared to two of 23 patients in group B. For patients who survived day 100 no difference in either the incidence or extent of chronic GVHD was observed. Thus, three of seven evaluable patients in group A developed chronic GVHD (extensive in all cases) as compared to 11 of 22 patients in group B (extensive in three patients and limited in the remaining eight patients).

Table 3 Patient outcome

In both groups, patients who received T cell add-back post SCT were at high risk for the development of extensive chronic GVHD. Thus, four of five patients who received a donor T cell infusion following the transplant subsequently developed extensive chronic GVHD. One patient (UPN3) died of progressive GVHD at 12 months following SCT and 2 months following infusion of 3 × 106/kg donor T cells for relapsed CML. The remaining patients have all required prolonged treatment with prednisolone and cyclosporine.

Infective complications

Most of the deaths in group A occurred secondary to infection (Table 3). Thus, at 12 months six of 11 patients in group A died secondary to infection (CMV disease n = 2, invasive pulmonary aspergillosis (IPA) n = 1, RSV/measles pneumonits n = 1, Legionella n = 1, E. coli sepsis n = 1) compared to only one of 23 patients (adenovirus) in group B (P = 0.002).

The propensity to infection in group A is also highlighted by the greater number of documented serious infections post day 21 in group A than in group B (Table 4). Thus, with a median follow-up of 28 months, four of 11 patients in group A had three or more significant infections as compared to one of 23 in group B (P = 0.03). Furthermore, patients in group A were more likely to have co-existent infections, with six of 11 patients in group A having two or more simultaneous infections as compared to only two of 23 patients in group B (P = 0.005). In a number of patients from group A, infection progressed despite the appropriate anti-microbial therapy. Patients UPN2 and UPN6 both developed IPA which failed to respond to conventional, and then liposomal amphotericin therapy. Patient UPN2 also developed a large, deep skin ulcer secondary to HSV II (proven on culture and biopsy), which failed to respond to aciclovir, foscarnet or cidofovir. Patient UPN5 developed CMV colitis and pneumonitis despite pre-emptive therapy with foscarnet and cidofovir and subsequent therapy with ganciclovir and intravenous immunoglobulin.

Table 4 Infectious complicationsa

There were no significant differences between the two groups in terms of the frequency of CMV reactivation in that five of six CMV seropositive individuals in group A and 11 of 19 in group B met the criteria for pre-emptive treatment for CMV reactivation or CMV disease. The median total duration of anti-CMV therapy administered was 98 days (range 8–127 days) in group A and 33 days in group B (range 13–129 days). Two patients from group A died from CMV disease in group A and none from group B.

Immune reconstitution

Group A patients showed a significant delay in the recovery of the absolute lymphocyte count following transplant. Thus, at 2 months post transplant the absolute lymphocyte count was >1.0 × 109/l in none of nine evaluable patients in group A but 10 of 21 patients in group B (P = 0.01). Absolute lymphocyte numbers for the first 5 months for both groups are shown in Figure 1. Analysis of T cell subsets at our centre is usually performed at 3 monthly intervals post transplant. The poor outcome of patients in group A meant that the majority were not evaluated and thus an evaluation of T cell subset reconstitution was not possible.

Figure 1
figure1

Absolute lymphocyte counts post-transplant. Mean ± s.e.m. absolute lymphocyte counts (×109/l) post transplant are given for group A (clear bars) and B (shaded bars). The number of patients in each group for each time point are as follows: 1 month – A 11, B 22; 2 months – A 8, B 20; 3 months – A 6, B 19; 4 months – A 6, B 19; 5 months – A 5, B 15. Significant differences between group A and B were observed at 2 months (P = 0.005), 3 months (P = 0.007) and 4 months (P < 0.05).

Overall survival and transplant-related mortality

With a median follow-up of 28 months the overall survival (OS) and TRM for all 36 patients at 12 months were 64.7% and 33.0%, respectively. However, there was a major difference in OS between the groups (Figure 2). Thus, at 12 months OS was 36.4% for group A and 78.8% for group B (P = 0.001). This difference was accounted for to a great extent by the high number of procedure-related deaths in group A (Figure 3). At 12 months the TRM was 63.6% in group A and 18.0% in group B (P = 0.003). The causes of procedure-related deaths for the whole group are shown in Table 3. The median time for procedure-related death in group A was 87 days (range 28–221 days) and in group B was 184.5 days (range 74–287 days). All but one of the procedure-related deaths in group A were secondary to infection.

Figure 2
figure2

Overall survival. Group A shown as dotted line and group B as solid line.

Figure 3
figure3

Transplant-related mortality. Group A shown as dotted line and group B as solid line.

Discussion

This report highlights important differences in the outcome of HLA-identical sibling SCT following two approaches to in vitro TCD, using either positive selection of CD34+ cells from PBSC or CAMPATH 1M treatment of bone marrow. Patients in both treatment groups received additional in vivo TCD, identical conditioning and no post-transplant immunosuppression. We found that allogeneic SCT using PBSC heavily depleted of T cells by immuno-magnetic selection of CD34+ cells, was associated with prolonged lymphocytopenia and an extremely high number of opportunistic infections leading to a high rate of procedure-related deaths. In contrast, allogeneic SCT using in vitro CAMPATH 1M treatment of bone marrow was associated with a lesser depletion of T cells, fewer infections and more rapid immune reconstitution. Both strategies were effective at preventing acute GVHD, and excluding those patients who received donor lymphocytes post transplant, at preventing extensive chronic GVHD. There was a low risk of graft rejection in both groups. Since the follow-up is relatively short and there are too few long-term survivors in group A, no conclusions can be made regarding the risk of relapse.

It seems likely that the overall determinant of clinical outcome in this report was degree of T cell depletion. There are five important qualifications to this statement. First, the type of graft differed in each group, such that patients in group A received PBSC and patients in group B received bone marrow. Thus, there may have been both quantitative (eg the number of CD34+ cells or CD34+CD38+ lymphocyte progenitors)27 and qualitative differences (eg the balance between Th1 or Th2 T cells)28 that could have conceivably affected outcome. However, to our knowledge, there are no substantive clinical data to support the contention that these differences could account for such a poor outcome in the PBSC arm. Second, exact comparison of the degree of TCD for the two methods is difficult since following CD34+ cell selection nearly all the remaining T cells will be viable whereas this may not be the case following in vitro treatment with CAMPATH 1M.29 Indeed, antibody coated T cells may undergo further lysis when they encounter fresh complement following infusion. Furthermore, pre-transplant in vivoCAMPATH 1-H treatment could have resulted in further destruction of donor T cells in the recipient and it is conceivable that there are differences between the two modes of TCD in terms of the sensitivity of the remaining T cells to in vivo lysis. It seems unlikely, however, that these factors could account for the >2 log difference in T cell dose observed. Third, although CD34+ cell selection removes most other cell types, CAMPATH 1M may be less efficient at removing certain cells such as NK cells (our unpublished observations) that could be of benefit to the patient post transplant. However, there are few clinical data available that clearly define the effect of changing the number of NK cells or other non-T cells in the graft upon the post-transplant course. Fourth, this was not a randomised study and ‘clusters’ can arise due to local or seasonal factors, which make an assessment of the reasons for differences between the two groups problematic. Finally, the patients in the two groups may have differed in certain characteristics that biased our evaluation of outcome. Although there were some differences between the groups in terms of the spectrum of diseases treated, group B had more high risk patients and included three patients who had received a previous transplant, suggesting any bias was likely to favour the null hypothesis that there was no difference in outcome between the groups.

All of the above factors may have had the potential to influence outcome, but one of the key differences between the groups was the measured number of T cells administered with the graft. A ‘safe’ threshold could be defined as the minimum T cell dose that permits high rates of engraftment, low rates of GVHD and low rates of serious infection. Any such level will be heavily influenced by patient factors such as age or status, donor characteristics including the degree of HLA disparity or CD34+ cell dose, and by other transplant factors such as the use of in vivo TCD or post-transplant immunosuppression. For example, there is abundant evidence that ‘safe’ T cell doses as defined for TCD HLA-identical sibling SCTs are less safe in the context of unrelated or mismatched transplants with resulting high rates of graft failure or infection.12,30,31

Using this protocol, our data suggest that extensive TCD of PBSC was associated with a very low risk of GVHD, high rates of engraftment but severely compromised immune reconstitution. There is little consistent information regarding the ‘safe’ T cell threshold in terms of immune competence of the recipient following HLA-identical sibling SCT. Recent studies indicate that infections cause death in less than 10% of patients following standard T cell-replete SCT, with no difference between recipients of peripheral blood stem cells or bone marrow.32,33 In an early study of 31 patients, intensive TCD of bone marrow by an E-rosette technique and administration of a fixed T cell dose of 0.1 × 106 T cells/kg was associated with only three infective deaths.11 Low rates of infection were also observed in a recent study of 14 patients who received CD34+-enriched bone marrow cells and a mean T cell dose of 0.09 × 106/kg. For the most part, other studies in the HLA-identical sibling transplant setting suggest that lesser degrees of TCD (T cell doses >0.1 × 106 T cells/kg) are associated with no major increase in the risk of opportunistic infection,6,34,35,36 although there are exceptions to this.37 The effect of additional in vivo TCD is unclear. In a retrospective analysis of two approaches, the addition of in vivo CAMPATH 1G to a protocol using in vitro TCD of the graft using CAMPATH 1M was not associated with higher rates of serious infection.38 Our report suggests that in adults, depletion of PBSC to 0.02 × 106 T cells/kg cells in combination with in vivo TCD using CAMPATH 1H results in prohibitively high rates of opportunistic infection following HLA-identical sibling SCT, that offsets any benefit in terms of GVHD reduction. Patients suffered considerable morbidity with more infections, more simultaneous infections and sub-optimal responses to anti-microbial therapy. Since initial immune reconstitution in adults is primarily dependent upon thymus-independent expansion of peripheral memory T cells from the graft,17 a major reduction in their number results in the loss of certain pathogen-specific immune responses.39 Excessive TCD, as reported here, appears to reduce the peripheral mature T cell pool below a critical threshold that ensures adequate resistance of the recipient to infection. Although, thymic output of naive T cells may eventually increase the T cell repertoire, its contribution is age-dependent and is likely to be delayed in adult patients.14,40 Thus, it is possible that younger patients may tolerate greater degrees of TCD without an unacceptable risk of infection.

One potential strategy to overcome the disadvantages of extensive TCD is the ‘add-back’ of T cells at a fixed dose at the time of transplant. It is possible that T cell doses lower than 1 × 106/kg could be administered safely following transplant if in vivo TCD using serotherapy was omitted from the conditioning regimen. However, the optimal timing and dose of T cell add back post transplant are unknown at present but will be influenced by the anticipated risks of GVHD or relapse at any one point post transplant. Other patients who may benefit from early add-back of T cells are those at risk of CMV re-activation or those who have persistent uncontrolled infections. It is possible that specific markers of immune reconstitution, such as thymic output of naive T cells41 may provide a better guide to patients who are most likely to benefit from such a strategy. An alternative approach might be to use in vitro TCD of PBSC using CAMPATH 1H, which is reported to be effective in permitting engraftment and preventing GVHD.42

In conclusion, we have reported the adverse effect of extensive in vitro TCD of PBSC upon TRM following HLA-identical sibling SCT. There was an unacceptably high risk of opportunistic infection in adult patients receiving CD34+ selected cells from peripheral blood and a mean T cell dose of 0.02 × 106 cells/kg which resulted in a considerable morbidity and which was responsible for 85% of procedure-related deaths. It is possible that the use of additional in vivo TCD with CAMPATH 1H further reduced the effective T cell dose. These results suggest that consideration should be given to fixed dose donor T cell add-back in patients who have received PBSC grafts which are extensively (>5 log) T cell depleted or that measures taken to achieve in vivo TCD are omitted.

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Acknowledgements

We would like to thank Nikki McKaeg, Judith Stuart and Brenda Miller for help in the collection and analysis of data. We would also like to thank Professor Paul Moss and Dr Charlie Craddock for their helpful criticisms regarding the manuscript.

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Correspondence to S Mackinnon.

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Chakraverty, R., Robinson, S., Peggs, K. et al. Excessive T cell depletion of peripheral blood stem cells has an adverse effect upon outcome following allogeneic stem cell transplantation. Bone Marrow Transplant 28, 827–834 (2001). https://doi.org/10.1038/sj.bmt.1703248

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Keywords

  • CD34 selection
  • allogeneic transplantation
  • T cell depletion

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