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Progenitor Cell Mobilisation

Trafficking of CD34+ cells into the peripheral circulation during collection of peripheral blood stem cells by apheresis

Bone Marrow Transplantation volume 28, pages 649–656 (2001)Cite this article

Abstract

The number of CD34+ cells collected during apheresis is related to the volume of blood processed. In large-volume apheresis (LVL) procedure, more cells can be collected than were originally present in the peripheral blood at the start of the collection procedure. We prospectively studied the levels of CD34+ cells in the blood and apheresis product during LVL procedures for 21 patients with acute myelogenous leukemia or multiple myeloma. These patients experienced a slow decline in blood CD34+ cell concentrations during the apheresis procedure. No patient demonstrated a sustained rise in CD34+ cell counts as a result of the procedure. The number of CD34+ cells collected exceeded the number calculated to be in the peripheral blood at the start of the procedure by an average of 3.0-fold. The efficiency of collection for CD34+ cells averaged 92.6% and did not vary with speed of blood processing, diagnosis, or mobilization regimen. The calculated release of CD34+ cells from other reservoirs into the peripheral blood averaged 3.71 × 106/min (range, 0.36–13.7 × 106/min), and correlated (r = 0.82) with the concentration of these cells in the peripheral blood at the start of the procedure. These data show that the apheresis procedure used in this study does not affect the release of CD34+ cells in a cytokine-treated patient. LVL will result in collection of larger quantities of CD34+ cells than procedures involving processing of smaller volumes of blood, but the number of cells collected is limited by the rate of release of these cells into the peripheral circulation where they are accessible for collection. Bone Marrow Transplantation (2001) 28, 649–656.

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Acknowledgements

The authors wish to thank the staff of the Apheresis Unit for their diligence in obtaining the multiple blood samples required for this investigation, and of the Clinical Cryobiology Laboratory and Hematology Laboratory for performing the analyses of these samples. Funding for this project was provided in parts by grants number CA18029 and CA15704 from the National Cancer Institute, Bethesda, MD.

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  1. Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

    SD Rowley, J Yu, T Gooley, S Heimfeld, L Holmberg, D Maloney & WI Bensinger

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  1. SD Rowley
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  2. J Yu
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  3. T Gooley
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Rowley, S., Yu, J., Gooley, T. et al. Trafficking of CD34+ cells into the peripheral circulation during collection of peripheral blood stem cells by apheresis. Bone Marrow Transplant 28, 649–656 (2001). https://doi.org/10.1038/sj.bmt.1703217

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