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Progenitor Cell Mobilisation

Chemotherapy for mobilisation of Ph-negative progenitor cells from patients with CML: impact of different mobilisation regimens

Bone Marrow Transplantation volume 27, pages 1125–1132 (2001)Cite this article

Abstract

Mobilised peripheral blood stem cells are widely used for autografting in patients with chronic myeloid leukaemia (CML) and it is generally thought that a high proportion of Ph-negative progenitor cells in the graft is desirable. We report here the results of 91 stem cell mobilisations performed with various chemotherapy regimens followed by G-CSF. We show that mobilisation of Ph-negative cells is possible after diagnosis as well as in advanced stages of the disease. The yield of Ph-negative cells is highly dependent on the chemotherapy regimen: while the combination of idarubicin and cytarabin for 3–5 days (IC3–5) mobilised Ph-negative cells in most patients, high-dose cyclophosphamide was ineffective. Mobilisation of Ph-negative progenitor cells after IC3 was at least as effective as after IC5; however, less apheresis sessions were required, and toxicity was much reduced after IC3. Compared to historical controls, IC was equally effective as the widely used ICE/minilCE (idarubicin, cytarabin, etoposide) protocol. No correlation was found between graft quality and the cytogenetic response to subsequent treatment with interferon-α. We conclude that IC3 is an effective and well-tolerated regimen for mobilising Ph-negative cells that compares well with more aggressive approaches such as IC5 and ICE/minilCE. Bone Marrow Transplantation (2001) 27, 1125–1132.

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References

  1. Goldman JM . Optimizing treatment for chronic myeloid leukemia New Engl J Med 1997 337: 270–271

    Article  CAS  PubMed  Google Scholar 

  2. Italian cooperative study group on CML . Interferon alfa-2a as compared with conventional chemotherapy for the treatment of chronic myeloid leukemia. The Italian Cooperative Study Group on Chronic Myeloid Leukemia New Engl J Med 1994 330: 820–825

    Article  Google Scholar 

  3. Buckner CD, Clift RA, Fefer A et al. Treatment of blastic transformation of chronic granulocytic leukemia by high dose cyclophosphamide, total body irradiation and infusion of cryopreserved autologous marrow Exp Hematol 1974 2: 138–146

    CAS  PubMed  Google Scholar 

  4. Goldman JM . Autografting cryopreserved buffy coat cells for chronic granulocytic leukaemia in transformation Exp Hematol 1979 7: (Suppl. 5) 389–397

    PubMed  Google Scholar 

  5. McGlave PB, De Fabritiis P, Deisseroth A et al. Autologous transplants for chronic myelogenous leukaemia: results from eight transplant groups Lancet 1994 343: 1486–1488

    Article  CAS  PubMed  Google Scholar 

  6. Finney R, McDonald GA, Baikie AG et al. Chronic granulocytic leukaemia with Ph 1 negative cells in bone marrow and a ten year remission after busulphan hypoplasia Br J Haematol 1972 23: 283–288

    Article  CAS  PubMed  Google Scholar 

  7. Cunningham I, Gee T, Dowling M et al. Results of treatment of Ph′+ chronic myelogenous leukemia with an intensive treatment regimen (L-5 protocol) Blood 1979 53: 375–395

    CAS  PubMed  Google Scholar 

  8. Petzer AL, Eaves CJ, Barnett MJ et al. Selective expansion of primitive normal hematopoietic cells in cytokine-supplemented cultures of purified cells from patients with chronic myeloid leukemia Blood 1997 90: 64–69

    CAS  PubMed  Google Scholar 

  9. Chalmers EA, Franklin IM, Kelsey SM et al. Treatment of chronic myeloid leukaemia in first chronic phase with idarubicin and cytarabine: mobilization of Philadelphia-negative peripheral blood stem cells Br J Haematol 1997 96: 627–634

    Article  CAS  PubMed  Google Scholar 

  10. Carella AM, Cunningham I, Lerma E et al. Mobilization and transplantation of Philadelphia-negative peripheral-blood progenitor cells early in chronic myelogenous leukemia J Clin Oncol 1997 15: 1575–1582

    Article  CAS  PubMed  Google Scholar 

  11. Fischer T, Neubauer A, Mohm J et al. Outcome of peripheral blood stem cell mobilization in advanced phases of CML is dependent on the type of chemotherapy applied Ann Hematol 1998 77: 21–26

    Article  CAS  PubMed  Google Scholar 

  12. Sahar E, Latt SA . Enhancement of banding patterns in human metaphase chromosomes by energy transfer Proc Nat Acad Sci USA 1978 75: 5650–5654

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Seong DC, Kantarjian HM, Ro JY et al. Hypermetaphase fluorescence in situ hybridization for quantitative monitoring of Philadelphia chromosome-positive cells in patients with chronic myelogenous leukemia during treatment Blood 1995 86: 2343–2349

    CAS  PubMed  Google Scholar 

  14. Schulze E, Krahl R, Thalmeier K et al. Detection of bcr-abl mRNA in single progenitor colonies from patients with chronic myeloid leukemia by PCR: comparison with cytogenetics and PCR from uncultured cells Exp Hematol 1995 23: 1649–1654

    CAS  PubMed  Google Scholar 

  15. Bhatia R, Verfaillie CM, Miller JS et al. Autologous transplantation therapy for chronic myelogenous leukemia Blood 1997 89: 2623–2634

    CAS  PubMed  Google Scholar 

  16. Tringali S, Santoro A, Scime R et al. High-dose cyclophosphamide for mobilization of circulating stem cells in chronic myeloid leukemia Eur J Haematol 1994 53: 1–5

    Article  CAS  PubMed  Google Scholar 

  17. Bhatia R, Verfaillie CM, Miller JS et al. Autologous transplantation therapy for chronic myelogenous leukemia Blood 1997 89: 2623–2634

    CAS  PubMed  Google Scholar 

  18. Carlo Stella C, Mangoni L, Almici C et al. Autologous transplant for chronic myelogenous leukemia using marrow treated ex vivo with mafosfamide Bone Marrow Transplant 1994 14: 425–432

    CAS  PubMed  Google Scholar 

  19. Deisseroth AB, Zu Z, Claxton D et al. Genetic marking shows that Ph+ cells present in autologous transplants of chronic myelogenous leukemia (CML) contribute to relapse after autologous bone marrow in CML Blood 1994 83: 3068–3076

    CAS  PubMed  Google Scholar 

  20. Carella A, Lerma E, Corsetti MT et al. Autografting with Philadelphia chromosome-negative mobilized hematopoietic progenitor cells in chronic myelogenous leukemia Blood 1999 93: 1534–1539

    CAS  PubMed  Google Scholar 

  21. Carella AM, Lerma E, Celesti L et al. Effective mobilization of Philadelphia-chromosome-negative cells in chronic myelogenous leukaemia patients using a less intensive regimen Br J Haematol 1998 100: 445–448

    Article  CAS  PubMed  Google Scholar 

  22. Carella AM, Podesta M, Frassoni F et al. Collection of ‘normal’ blood repopulating cells during early hemopoietic recovery after intensive conventional chemotherapy in chronic myelogenous leukemia Bone Marrow Transplant 1993 12: 267–271

    CAS  PubMed  Google Scholar 

  23. Fischer T, Neubauer A, Mohm J et al. Chemotherapy-induced mobilization of karyotypically normal PBSC for autografting in CML Bone Marrow Transplant 1998 21: 1029–1036

    Article  CAS  PubMed  Google Scholar 

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  1. Department of Hematology, University of Leipzig, Johannisallee 32, Leipzig, 04103, Germany

    M Deininger

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  2. W Pönisch
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for the East German Study Group Haematology/Oncology (OSHO)

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Deininger, M., Pönisch, W., Krahl, R. et al. Chemotherapy for mobilisation of Ph-negative progenitor cells from patients with CML: impact of different mobilisation regimens. Bone Marrow Transplant 27, 1125–1132 (2001). https://doi.org/10.1038/sj.bmt.1703057

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