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Immunobiology

Oligoclonal expansion of CD4+CD28− T lymphocytes in recipients of allogeneic hematopoietic cell grafts and identification of the same T cell clones within both CD4+CD28+ and CD4+CD28− T cell subsets

Bone Marrow Transplantation volume 27, pages 1095–1100 (2001)Cite this article

Abstract

Recipients of allogeneic bone marrow grafts have clonally expanded CD8+CD28− T lymphocytes during the early period after transplantation, which leads to skewing of T cell receptor (TCR) repertoires. Here, we have addressed the question of whether clonal expansion of CD28− T cells is also observed in CD4+ T lymphocytes after human allogeneic hematopoietic cell transplantation. We found that the fraction of T cells lacking CD28 expression in the CD4+ subset was increased after transplantation, and expanded CD4+CD28− T lymphocytes carrying certain TCRBV subfamilies showed limited TCR diversity. In order to further study the ontogeny of CD4+CD28− T cells, we analyzed the complementarity-determining region 3 (CDR3) of the TCR-β chain of CD4+CD28+ and CD4+CD28− cells. We identified the same T cell clones within both CD4+CD28− and CD4+CD28+ T cell subsets. These results suggest that both subsets are phenotypic variants of the same T cell lineage. Bone Marrow Transplantation (2001) 27, 1095–1100.

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Acknowledgements

We are grateful to the hematology staff at Akita University Medical Center for their treatment of the patients in this study. This work was supported by grants from the Ministry of Education, Science, Sports and Culture of Japan (Grant No. 08670508, 10670932), the Yamashita Taro-Kensho Memorial Foundation and the Uehara Memorial Foundation.

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Authors and Affiliations

  1. Department of Internal Medicine III, Akita University School of Medicine, Akita, Japan

    M Hirokawa, T Horiuchi, Y Kawabata, A Kitabayashi, H Saitoh, Y Ichikawa & AB Miura

  2. Department of Immunology, Shionogi Institute for Medical Science, Osaka, Japan

    T Matsutani, T Yoshioka, Y Tsuruta & R Suzuki

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Hirokawa, M., Horiuchi, T., Kawabata, Y. et al. Oligoclonal expansion of CD4+CD28− T lymphocytes in recipients of allogeneic hematopoietic cell grafts and identification of the same T cell clones within both CD4+CD28+ and CD4+CD28− T cell subsets. Bone Marrow Transplant 27, 1095–1100 (2001). https://doi.org/10.1038/sj.bmt.1703045

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