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Progenitor Cell Expansion

The CD34+CD38neg population is significantly increased in haemopoietic cell expansion cultures in serum-free compared to serum-replete conditions: dissociation of phenotype and function

Bone Marrow Transplantation volume 27, pages 365–371 (2001)Cite this article

Abstract

Expansion of haemopoietic stem cells is proposed to combat graft failure in adult recipients following cord blood (CB) transplantation. Cultures are traditionally performed in medium containing FCS, but to transfer expansion to the clinic, ‘good manufacturing practice’ (GMP) standards are required. This study evaluated expansion cultures in culture bags and serum-free (SF) conditions, to comply with GMP, by analysing sub-populations of CD34+ cells, colony-forming cells (CFC) and long-term culture initiating cells (LTC-IC). CD34+cell analysis has previously been used to measure clonogenic capacity and the CD34+CD38neg surface phenotype to measure primitive cell numbers. In this study, comparison of expansion in serum-replete medium with that in SF conditions demonstrated a lack of expression of CD38 on CD34+ cells in the absence of serum. These findings must be considered in clinical studies using in vitro expansion in SF conditions, and the CD34+CD38neg phenotype should not be used to confirm maintenance, or expansion, of primitive progenitor cells. Bone Marrow Transplantation (2001) 27, 365–371.

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Acknowledgements

This work was funded by the Leukaemia Research Fund grant 98/47. We thank the midwives on Delivery Suite, Southmead Hospital, all mothers who donated CB for the project and Graham Molineux (Amgen) for the gift of growth factors, ‘Modified Ex-vivo Expansion Medium’ and culture bags.

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  1. Division of Transplantation Sciences, University of Bristol, Southmead Hospital, Bristol, UK

    C Donaldson, P Denning-Kendall, B Bradley & J Hows

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  1. C Donaldson
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Donaldson, C., Denning-Kendall, P., Bradley, B. et al. The CD34+CD38neg population is significantly increased in haemopoietic cell expansion cultures in serum-free compared to serum-replete conditions: dissociation of phenotype and function. Bone Marrow Transplant 27, 365–371 (2001). https://doi.org/10.1038/sj.bmt.1702810

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