Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Technical Report
  • Published:

High-efficiency volume reduction of cord blood using pentastarch

Bone Marrow Transplantation volume 27, pages 457–461 (2001)Cite this article

Abstract

Human umbilical cord blood (UCB) has been used successfully to treat a variety of genetic, hematological, and oncologic disorders. However, the low number of hematopoietic progenitor cells available in donated cord blood samples limits transplantation of cord blood to children and small adults. Reduction of the volume of umbilical cord blood is widely used in cord blood banking to reduce the storage requirements in large-scale UCB banks. Unfortunately, during the volume reduction process, up to 40% or more of the progenitor cells are lost using current reduction methods. This study describes a highly reproducible, double collection technique using Pentaspan to reduce UCB volume by red cell depletion. This results in the preservation of critical hematopoietic progenitor cells. The final volume of the leukocyte concentrates (LC) was 19.8 ± 0.4 ml with 95% red cell depletion. The recovery of nucleated cells (NC), mononuclear cells (MNC), CD34+ cells and colony-forming units (CFU) following double collection was 89%, 94%, 96%, and 106%, respectively. This is significantly higher than the recovery from single collections, where recovery was 74%, 77%, 84%, and 91% for NC, MNC, CD34+ and CFU, respectively. The double collection technique provides an efficient and highly reproducible method for the preparation of UCB for long-term storage and transplantation. Bone Marrow Transplantation (2001) 27, 457–461.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Broxmeyer HE, Gordon GW, Hangoc G et al. Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells Proc Natl Acad Sci USA 1989 86: 3828–3832

    Article  CAS  Google Scholar 

  2. Broxmeyer HE, Hangoc G, Cooper S et al. Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults Proc Natl Acad Sci USA 1992 89: 4109–4113

    Article  CAS  Google Scholar 

  3. Vaziri H, Dragowska W, Allsop RC et al. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age Proc Natl Acad Sci USA 1994 91: 9857–9860

    Article  CAS  Google Scholar 

  4. Rocha V, Wagner JE, Sobocinski KA et al. Graft-versus-host disease in children who have received a cord-blood or bone marrow transplant from an HLA-identical sibling New Engl J Med 2000 342: 1846–1854

    Article  CAS  Google Scholar 

  5. Donaldson C, Armitage WJ, Laundy V et al. Impact of obstetric factors on cord blood donation for transplantation Br J Haematol 1999 106: 128–132

    Article  CAS  Google Scholar 

  6. Rubinstein P, Dobrila L, Richard E et al. Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution Proc Natl Acad Sci USA 1995 92: 10119–10122

    Article  CAS  Google Scholar 

  7. Perutelli P, Gatellani S, Scarso L et al. Processing of human cord blood by three different procedures for red blood cell depletion and mononuclear cell recovery Vox Sang 1999 76: 237–240

    Article  CAS  Google Scholar 

  8. Fraser J, Cairo MS, Wagner E et al. Cord blood transplantation study (COBLT): cord blood bank standard operating procedures J Hematother 1998 7: 521–561

    Article  CAS  Google Scholar 

  9. Sousa T, De Sousa ME, Godinho MI et al. Umbilical cord blood processing: volume reduction and recovery of CD34+ cells Bone Marrow Transplant 1997 19: 311–313

    Article  CAS  Google Scholar 

  10. Gluckman E, Rocha V, Boyer-Chammard A et al. Outcome of cord blood transplantation from related and unrelated donors New Engl J Med 1997 337: 373–381

    Article  CAS  Google Scholar 

  11. Keeney M, Chin-Yee I, Weir K et al. Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines Cytometry 1998 34: 61–70

    Article  CAS  Google Scholar 

  12. Haugland RP . Handbook of Fluorescent Probes and Research Chemicals Molecular Probes: Eugene, OR 1996

    Google Scholar 

  13. Brown RI . The physics of continuous flow centrifugal cell separation Artificial Organs 1989 13: 4–20

    Article  CAS  Google Scholar 

  14. Akabutu J, Yang H, McGann LE . Centralized public banking of umbilical cord blood stem cells: a Canadian perspective Blood 1999 94: (Suppl. 1) 10 (Abstr. 2538)

    Google Scholar 

  15. Trivedi SM, Humphrey RL, Braine HD et al. Hydroxyethyl starch serum levels in leukapheresis donors measured by modified periodic acid-Schiff staining technique Transfusion 1984 24: 260–263

    Article  CAS  Google Scholar 

  16. Lee JH, Leitman SF, Klein HG . A controlled comparison of the efficacy of hetastarch and pentastarch in granulocyte collections by centrifugal leukapheresis Blood 1995 86: 4664–4666

    Google Scholar 

  17. Strauss RG . In vitro comparison of the erythrocytes sedimenting properties of dextran, hydroxyethyl starch and a new low-molecular weight hydroxyethyl starch Vox Sang 1979 37: 268–271

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by the Dr Charles Allard Foundation and a grant in aid from the Western Economic Partnership Agreement (Alberta Health and Wellness, Government of Alberta and Western Diversification, Government of Canada), 1999.

Author information

Authors and Affiliations

  1. Alberta Cord Blood Bank, Edmonton, Alberta, Canada

    H Yang, D Abley, LE McGann & J Akabutu

  2. Canadian Blood Services, Edmonton, Alberta, Canada

    H Yang & LE McGann

  3. Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada

    JP Acker & LE McGann

  4. Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada

    J Akabutu

Authors
  1. H Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. JP Acker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D Abley
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. LE McGann
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J Akabutu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, H., Acker, J., Abley, D. et al. High-efficiency volume reduction of cord blood using pentastarch. Bone Marrow Transplant 27, 457–461 (2001). https://doi.org/10.1038/sj.bmt.1702807

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.bmt.1702807

Keywords

Search

Advanced search

Quick links