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Immune Reconstitution

Allogeneic peripheral blood stem cell transplantation results in less alteration of early T cell compartment homeostasis than bone marrow transplantation

Bone Marrow Transplantation volume 27, pages 167–175 (2001)Cite this article

Abstract

Since low T cell counts evaluated 1 month after allogeneic bone marrow transplantation (BMT) are associated with an increased risk of leukemia relapse (Powles et al., Blood 1998; 91: 3481–3486), we compared, in a randomized multicentric clinical study, the peripheral blood cells obtained 30 days after allogeneic BMT vs allogeneic G-CSF-mobilized peripheral blood stem cell transplantation (BCT) in an HLA-identical setting. T cell counts were higher 30 days after BCT (718 ± 142 cells/μl, n = 20) than after BMT (271 ± 53 cells/μl, n = 26, P = 0.006). However, T cells were less activated after BCT than after BMT, as demonstrated by a lower expression level of CD25 and a lower percentage of HLA-DR+ and CD95+ T cells. Furthermore, CD4+, CD8+and CD45RA+ post-BCT T cell counts correlated with the number of cells infused with the PBSC graft, while such a correlation was not observed between post-BMT counts and BM graft cell numbers, suggesting that the intensity of post-transplant peripheral lymphoid expansion and/or deletion differed between BCT and BMT. A comparison of the input of T cells expressing different CD45 isoforms with the post-transplant cell recovery further confirmed that, within the CD4+ T cell subset, post-transplant expansions occurred at a higher level after BMT than after BCT, affecting mainly the CD4+ CD45RO+subset. Altogether, our data demonstrate for the first time in a randomized setting that homeostasis of the T cell pool is less altered early after BCT than after BMT. This may have a strong impact on the graft-versus-leukemia (GVL) effect and subsequent relapse rate. Bone Marrow Transplantation (2001) 27, 167–175.

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Acknowledgements

We are indebted to all the clinical staff, cell therapy units and patients for their help and their contribution to this work, and namely Drs Attal M, Belhabri A, Boiron M, Bordigoni P, Bulabois CE, de Cervens C, Duval M, François S, Freycon F, Garban F, Guilhot F, Ifrah N, Jouet JP, Michallet M, Milpied N, Oriol P, Payen C, Pegourie B, Pico JL, Reiffers J, Rio B, Rohrlich P, Sadoun A, Sotto JJ, Sutton A, Vernant JP and Witz F. We also thank Mrs Joelle Beaune and Sandra Cournier for data management, Drs Gérard Socié and Anne Aupérin for critical and helpful reading of the manuscript and the Société Française de Greffe de Moelle for its support. This work was supported by grants from the Etablissement Français des Greffes, the Association pour la Recherche sur le Cancer and the Fondation de France.

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Authors and Affiliations

  1. Etablissement Français du Sang-Bourgogne/Franche-Comté, Besançon, France

    H Tayebi, P Tiberghien, C Ferrand, A Lienard, A Duperrier, P Saas, P Hervé & E Robinet for the Société Française de Greffe de Moelle

  2. Hematology Department, Hôpital Jean Minjoz, Besançon, France

    JY Cahn

  3. Unité de Medecine Transfusionelle et d'Hemovigilance, Institut Gustave Roussy, Villejuif, France

    V Lapierre

  4. Hematology Department, Hôpital Henri Mondor, Créteil, France

    M Kuentz

  5. Bone Marrow Transplantation Unit, Institut Paoli Calmettes, Marseille, France

    D Blaise

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Tayebi, H., Tiberghien, P., Ferrand, C. et al. Allogeneic peripheral blood stem cell transplantation results in less alteration of early T cell compartment homeostasis than bone marrow transplantation. Bone Marrow Transplant 27, 167–175 (2001). https://doi.org/10.1038/sj.bmt.1702753

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