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Transplantation of CD34-enriched peripheral stem cells from an HLA-haplotype mismatched donor to a patient with severe aplastic anemia

Bone Marrow Transplantation volume 27, pages 111–113 (2001)Cite this article

Abstract

A 14-year-old girl developed very severe aplastic anemia unresponsive to steroids, cyclosporine, ATG and filgrastim. She experienced repeated bacterial infections, hypermenorrhagia and epistaxis and received numerous transfusions. Lacking a matched family or unrelated donor, she was transplanted 6 months after diagnosis with CD34+cell-enriched peripheral stem cells from her HLA-haploidentical uncle. Conditioning included fludarabine, cyclophosphamide, 800 cGy TLI and OKT3. Prompt and sustained trilineage engraftment occurred. Acute GVHD grade 1 and herpes esophagitis were successfully treated. Eight months after grafting she was well with stable hematopoiesis. She then succumbed to fulminant hepatic failure due to adenovirus infection. Bone Marrow Transplantation (2001) 27, 111–113.

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References

  1. Bacigalupo A, Oneto R, Bruno B et al. Current results of bone marrow transplantation in patients with aquired severe aplastic anemia Acta Haematol 2000 103: 19–25

    Article  CAS  Google Scholar 

  2. Führer M, Burdach S, Ebell W et al. Relapse and clonal disease in children with aplastic anemia after immunosuppressive therapy: the SAA 94 experience Klin Pädiatr 1998 210: 173–179

    Article  Google Scholar 

  3. Sanders JE, Storb R, Anasetti C et al. Marrow transplant experience for children with severe aplastic anemia Am J Pediatr Hematol Oncol 1994 16: 43–49

    CAS  PubMed  Google Scholar 

  4. Wagner JL, Storb R . Allogeneic transplantation for aplastic anemia. In: Thomas ED, Blume KG, Forman SJ (eds) Hematopoietic Cell Transplantation Blackwell Science: Malden, MA 1999 791–806

    Google Scholar 

  5. Deeg HJ, Seidel K, Casper J et al. Marrow transplantation from unrelated donors for patients with severs aplastic anemia who have failed immunosuppressive therapy Biol Blood Marrow Transplant 1999 5: 243–252

    Article  CAS  Google Scholar 

  6. Tzeng CH, Chen PM, Fan S et al. CY/TBI as a pretransplant regimen for allogeneic bone marrow transplantation for severe aplastic anemia using HLA-haploidentical family donors Bone Marrow Transplant 1999 18: 273–277

    Google Scholar 

  7. Schumm M, Lang P, Taylor G et al. Isolation of highly purified autologous and allogeneic peripheral CD34+ cells using the CliniMACS device J Hematother 1999 8: 209–218

    Article  CAS  Google Scholar 

  8. Margolis DA, Casper JT . Alternative-donor hematopoietic stem-cell transplantation for severe aplastic anemia Semin Hematol 2000 37: 43–55

    Article  CAS  Google Scholar 

  9. Martin PJ, Hansen JA, Buckner CD et al. Effects of in vitro depletion of T cells in HLA-identical allogeneic marrow grafts Blood 1985 66: 664–672

    CAS  PubMed  Google Scholar 

  10. Aversa F, Fabilio A, Velardi A et al. Treatment of high risk acute leukemia with T-cell depleted stem cells from related donors with one fully mismatched HLA-haplotype New Engl J Med 1998 339: 1186–1193

    Article  CAS  Google Scholar 

  11. Schwinger W, Urban C, Lackner H et al. Unrelated peripheral blood stem cell transplantation with ‘megadoses’ of purified CD34+ cells in three children with refractory severe aplastic anemia Bone Marrow Transplant 2000 25: 513–517

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatric Hematology-Oncology and Endocrinology, West German Tumor Center, University Hospital, Essen, Germany

    B Kremens, O Basu & W Havers

  2. Department of Immunology, West German Tumor Center, University Hospital, Essen, Germany

    H Grosse-Wilde

  3. Department of Radiotherapy, West German Tumor Center, University Hospital, Essen, Germany

    W Sauerwein

  4. Department of Bone Marrow Transplantation, West German Tumor Center, University Hospital, Essen, Germany

    UW Schaefer

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  1. B Kremens
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  2. O Basu
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  3. H Grosse-Wilde
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  4. W Sauerwein
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  5. UW Schaefer
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  6. W Havers
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Kremens, B., Basu, O., Grosse-Wilde, H. et al. Transplantation of CD34-enriched peripheral stem cells from an HLA-haplotype mismatched donor to a patient with severe aplastic anemia. Bone Marrow Transplant 27, 111–113 (2001). https://doi.org/10.1038/sj.bmt.1702748

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  • DOI: https://doi.org/10.1038/sj.bmt.1702748

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