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Graft Versus Host Disease

Contribution of TNF-α and IL-10 gene polymorphisms to graft-versus-host disease following allo-hematopoietic stem cell transplantation

Bone Marrow Transplantation volume 26, pages 1317–1323 (2000)Cite this article

Abstract

Some cytokines are believed to play a role in the development of acute and chronic GVHD after allo-hematopoietic stem cell transplantation. It has been reported that TNF-α and IL-10 gene polymorphisms are associated with the production of those cytokines and the development of graft failure after organ transplantation and systemic lupus erythematosus. We examined whether TNF-α and IL-10 gene polymorphisms affect the severity of acute GVHD (aGVHD) and chronic GVHD (cGVHD). Sixty-two and 54 patients were available for the analysis of aGVHD and cGVHD, respectively. We analyzed the gene polymorphisms derived from pre- and post-transplant blood cells. Donor-derived TNF2 allele (A) was more frequently detected in patients with aGVHD III/IV than those aGVHD 0-II (2/6 vs 2/56) (P = 0.04). The donors of the patients with cGVHD more frequently possessed a greater number of alleles (allele 13 or more which contain 26 or more CA repeats) in IL-10.G than those without (13/26 vs 5/28) (P = 0.02), and the patients with cGVHD had more CA repeats in donor-derived IL-10.G than those without (mean = 25.2 vs 23.4) (P= 0.01). Donor-derived TNF-308 and IL-10.G alleles may contribute to severe aGVHD and cGVHD, respectively, and will help us distinguish those patients at high risk for GVHD. Bone Marrow Transplantation (2000) 26, 1317–1323.

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Acknowledgements

The authors thank Mrs M Sakaue for technical assistance.

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Authors and Affiliations

  1. First Department of Internal Medicine, College of Biochemical Technology, Niigata University School of Medicine, Niigata, Japan

    H Takahashi, N Suzuki, T Kuroha & Y Aizawa

  2. Division of Bone Marrow Transplantation, Niigata University Medical Hospital, Niigata, Japan

    T Furukawa & S Hashimoto

  3. Laboratory Division, Niigata University Medical Hospital, Niigata, Japan

    F Yamazaki & K Inano

  4. Department of Medical Technology, College of Biochemical Technology, Niigata University School of Medicine, Niigata, Japan

    M Takahashi

  5. Division of Blood Transfusion, Niigata University Medical Hospital, Niigata, Japan

    T Koike

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Takahashi, H., Furukawa, T., Hashimoto, S. et al. Contribution of TNF-α and IL-10 gene polymorphisms to graft-versus-host disease following allo-hematopoietic stem cell transplantation. Bone Marrow Transplant 26, 1317–1323 (2000). https://doi.org/10.1038/sj.bmt.1702724

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  • DOI: https://doi.org/10.1038/sj.bmt.1702724

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