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Post-Transplant Complications

Monitoring four herpesviruses in unrelated cord blood transplantation

Bone Marrow Transplantation volume 26, pages 1193–1197 (2000)Cite this article

Abstract

Cord blood transplantation, which has lower risk of graft-versus-host disease than bone marrow transplantation, might have higher risk of infections. A system to quantify four herpesviruses, CMV, human herpesvirus 6 (HHV6), EBV, varicella-zoster virus using the real-time PCR assay was established and applied for prospective viral load monitoring in three recipients undergoing cord blood transplantation. CMV and HHV6 were detected in peripheral blood from all three recipients, while EBV was detected in two. Varicella-zoster virus was not detected at all. At the peak of HHV6 or CMV, each patient showed virus-related symptoms. During the pre-transplant period, CMV DNA was detected in two recipients who later developed CMV-related diseases. These observations indicate that our system is not only useful for managing herpesviruses infections in transplant recipients, but also a powerful method for clarifying the relationships between the viral load and clinical symptoms. Bone Marrow Transplantation (2000) 26, 1193–1197.

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Acknowledgements

We thank Prof K Watanabe, Department of Pediatrics, Nagoya University School of Medicine, for helpful advice, and Prof K Yamanishi, Department of Microbiology, Osaka University School of Medicine, for a gift of PSTY05. This work was supported by a grant from Japan Society for the Promotion of Science (JSPS-RFTF97L00703) and also by a grant from Ministry of Health and Welfare, Health Science Research Grants, Research on Immunology, Allergy and Organ Transplantation (H11-Menneki-006).

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Authors and Affiliations

  1. Department of Pediatrics, Nagoya University School of Medicine, Nagoya, Japan

    N Tanaka, H Kimura, Y Hoshino, K Horibe & S Kojima

  2. Division of Hematology/Oncology, Children's Medical Center, Japanese Red Cross Nagoya First Hospital, Nagoya, Japan

    K Kato

  3. Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

    T Yoshikawa & Y Asano

  4. Department of Health Science, Nagoya University School of Medicine, Nagoya, Japan

    T Morishima

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  1. N Tanaka
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Tanaka, N., Kimura, H., Hoshino, Y. et al. Monitoring four herpesviruses in unrelated cord blood transplantation. Bone Marrow Transplant 26, 1193–1197 (2000). https://doi.org/10.1038/sj.bmt.1702710

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