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Graft Versus Host Disease

Significant MLR but not CTL responses against recipient antigens generated in T cells from bone marrow chimeras recovered from acute GVHD

Bone Marrow Transplantation volume 26, pages 1069–1076 (2000)Cite this article

Abstract

Lethally irradiated AKR mice received BMT from H-2D and minor lymphocyte stimulatory (Mls)-1 disparate B10.A mice. No GVHD signs were detected in AKR recipients of T cell-depleted BM cells (1 × 107) alone ([B10.A → AKR] T−). When B10.A splenic T cells (1 × 105) were injected in addition to T cell-depleted BM cells ([B10.A → AKR] T+), overt GVHD was observed. [B10.A → AKR] T+ chimeras recovered from the GVHD 8 weeks after BMT. In T cells from these [B10.A → AKR] T+ chimeras, a substantial population of Mls-1a-reactive Vβ6+ T cells was present, whereas the Vβ6+ cells were deleted in [B10.A → AKR] T− chimeras. T cells from [B10.A → AKR] T+ chimeras showed considerable MLR but no CTL response against AKR cells (split tolerance). Upon stimulation with AKR stimulators or anti-CD3 MoAb, T cells from [B10.A → AKR] T+ chimeras produced significantly more IL-4 but significantly less IFN-γ compared with those from [B10.A → AKR] T− chimeras or unmanipulated B10.A mice. The serum level of IgG1 in [B10.A → AKR] T+chimeras was also significantly higher than that in [B10.A → AKR] T− or B10.A mice. The present findings suggest that the split tolerance observed in BMT chimeras recovered from GVHD is attributable to the Th2 dominant state. Bone Marrow Transplantation (2000) 26, 1069–1076.

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Acknowledgements

We thank Ms Ryoko Hosohata and Kaori Kohno for their secretarial assistance. This study was supported in part by a Grant-in-Aid for Scientific Research by the Ministry of Education, Science, Sports and Culture, Japan, Research on Immunology, Allergy and Organ Transplantation, Ministry of Health and Welfare, Japan, Nishimura Aging Fund and The Tomakomai East Hospital Foundation.

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  1. Division of Immunobiology, Section of Pathophysiology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan

    T Morohashi, K Ogasawara, N Kitaichi, K Iwabuchi & K Onoé

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Morohashi, T., Ogasawara, K., Kitaichi, N. et al. Significant MLR but not CTL responses against recipient antigens generated in T cells from bone marrow chimeras recovered from acute GVHD. Bone Marrow Transplant 26, 1069–1076 (2000). https://doi.org/10.1038/sj.bmt.1702663

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