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Generation of HLA-A2 subtype specific cytotoxic T lymphocytes from cord blood used for cord blood stem cell transplantation

Bone Marrow Transplantation volume 26, pages 451–453 (2000)Cite this article

Abstract

Alloantigen reactive cytotoxic T lymphocytes (CTL) were generated from cord blood (CB) lymphocytes used for cord blood stem cell transplantation (CBSCT). The CTL were cytotoxic against the patient's leukemic cells, as well as the patient's EBV-lymphoblastoid cell line (EBV-LCL), and PHA blasts. The cytotoxicity against patient's EBV-LCL was blocked by anti-HLA-A2 MoAb, and anti-HLA-class I MoAb. The CTL recognized A*0206 positive EBV-LCLs, but not A*0201, A*0204, or A*0207 positive EBV-LCLs, suggesting that this CTL recognizes HLA-A*0206. This case suggests that CB T cells may be competent enough to generate CTL to induce a GVL effect, together with those against A*0206, in patients with CBSCT. Bone Marrow Transplantation (2000) 26, 451–453.

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Acknowledgements

We thank Mr Toru Kato for detecting the HLA-A2 allele of EBV-LCLs and Dr Kyogo Ito for providing MA2.1 moAb against HLA-A2. We also thank Mrs Etsuko Maruya for detecting HA-1 allele of LCLs and Mr Dee Lynn Johnson for his advice on this manuscript.

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Authors and Affiliations

  1. Department of Pediatrics, Nagoya City University Medical School, Nagoya, Japan

    M Yazaki, Y Ito, T Ito, C Mori & Y Wada

  2. Laboratory of Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan

    T Takahashi

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  1. M Yazaki
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Yazaki, M., Takahashi, T., Ito, Y. et al. Generation of HLA-A2 subtype specific cytotoxic T lymphocytes from cord blood used for cord blood stem cell transplantation. Bone Marrow Transplant 26, 451–453 (2000). https://doi.org/10.1038/sj.bmt.1702537

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