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Progenitor Cell Mobilisation

Mobilization kinetics of peripheral blood progenitor cells after IAPVP-16 salvage chemotherapy plus G-CSF in lymphoproliferative disorders

Bone Marrow Transplantation volume 26, pages 127–132 (2000)Cite this article

Abstract

We have explored the efficacy of salvage chemotherapy combination, IAPVP-16 (ifosfamide 5 g/m2 on day 1; VP-16 100 mg/m2 on days 1–3; ara-C 1.2 g/m2/12 h on days 1 and 2; methylprednisolone 80 mg/m2 on days 1–5) plus g-csf for pbpc mobilization. this protocol was used in 45 patients with relapsed or refractory lymphoproliferative diseases who underwent 85 leukaphereses. in 41 patients >2 × 106/kg CD34+ cells were obtained after a median of two procedures. The median number of CD34+ cells harvested was 3.2 × 106/kg per apheresis and 8.4 × 106/kg per patient. Seven of 10 patients who had failed previous mobilization attempts achieved more than 2 × 106 CD34+ cells/kg in a maximum of three aphereses. A history of previous mobilization failure and a low platelet count (<150 × 109/l) negatively influenced the CD34+ cell yield in univariate and multivariate analyses. A good correlation was found between the circulating CD34+cells/μl and the CD34+ cells and CFU-GM in the leukaphereses products (r = 0.93 and r = 0.73, P < 0.001), and ⩾17 cd34+cells/μl predicted the achievement of >2 × 106/kg CD34+ cells in a single leukapheresis in more than 90% of cases. IAPVP-16 plus G-CSF may be specially indicated in tandem transplantations or CD34+ selection and in patients who have failed previous mobilization attempts. Bone Marrow Transplantation (2000) 26, 127–132.

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Acknowledgements

This study was supported in part by grant FIS 97/0626 from the Fondo de Investigaciones Sanitarias del Ministerio de Sanidad y Consumo and a grant from Fundación Ramón Areces (JS/1997–1999).

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Authors and Affiliations

  1. Clinical Hematology Division, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain

    A Altés, R López, R Martino, L Muñoz, A Santamaría, G Perea, J Briones, A Salar, A Sureda, S Brunet & J Sierra

  2. Hemotherapy Service, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain

    C Martinez & P Madoz

  3. Cancer Research Institute, Autonomous University of Barcelona, Spain

    E Cabezudo

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  1. A Altés
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  2. R López
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Altés, A., López, R., Martino, R. et al. Mobilization kinetics of peripheral blood progenitor cells after IAPVP-16 salvage chemotherapy plus G-CSF in lymphoproliferative disorders. Bone Marrow Transplant 26, 127–132 (2000). https://doi.org/10.1038/sj.bmt.1702476

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