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Progenitor Cell Mobilization

Mobilisation of haemopoietic progenitors in CML: a second course of intensive chemotherapy does not improve Ph-negativity in stem cell harvests

Bone Marrow Transplantation volume 25, pages 1147–1155 (2000)Cite this article

Abstract

We collected peripheral blood stem cells (PBSC) in 19 early chronic phase CML patients following each of two consecutive cycles of intensive chemotherapy (CT) to evaluate whether an additional cycle of CT would increase Philadelphia (Ph)-negativity of the PBSC harvest. Autologous SCT (autoSCT) was performed if a major cytogenetic response (MCR) of the PBSC harvest was obtained. CT consisted of cytarabine 200 mg/ m2/day (days 1–7)/idarubicin 12 mg/m2/day (days 1–2) (cycle one) and cytarabine 2000 mg/m2/day (days 1–6)/amsacrine 120 mg/m2/day (days 1–3) (cycle two). One patient died of fungal pneumonia after the first cycle. Stem cells were harvested in 18 patients after cycle one and in 16 patients after cycle two. After the first cycle, all patients showed a cytogenetic response of their graft (MCR in eight patients: three complete, five partial), after cycle two, seven patients obtained an MCR (one complete, six partial). Seven patients became eligible for autoSCT. All patients proceeded with IFNα maintenance. Currently, 16 patients are alive. At the latest cytogenetic examination of bone marrow, four patients showed an MCR and four a minor response. In conclusion, although a second cycle of CT may contribute to elimination of leukemia residing in the patient, it appeared to be ineffective in improving the Ph-negativity of the PBSC graft. Bone Marrow Transplantation (2000) 25, 1147–1155.

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Authors and Affiliations

  1. Department of Haematology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands

    JJWM Janssen, G-J Schuurhuis & GJ Ossenkoppele

  2. Department of Haematology, Daniel den Hoed Cancer Centre, University Hospital, Rotterdam, The Netherlands

    RS van Rijn & JJ Cornelissen

  3. Department of Statistics-HOVON Data Centre, Daniel den Hoed Cancer Centre, University Hospital, Rotterdam, The Netherlands

    B van der Holt

  4. Department of Haematology, University Hospital, Groningen, The Netherlands

    E Vellenga

  5. Department of Haematology, University Hospital, Leuven, Belgium

    GEG Verhoef

  6. Department of Medical Genetics, University Hospital, Groningen, The Netherlands

    E van den Berg

  7. Department of Cytogenetics, University Hospital, Leuven, Belgium, The Netherlands

    A Hagemeijer

  8. Department of Cytogenetics, University Hospital, Rotterdam, The Netherlands

    R Släter

  9. Department of Cytogenetics, University Hospital Vrije Universiteit, Amsterdam, The Netherlands

    AWM Nieuwint

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Janssen, J., van Rijn, R., van der Holt, B. et al. Mobilisation of haemopoietic progenitors in CML: a second course of intensive chemotherapy does not improve Ph-negativity in stem cell harvests. Bone Marrow Transplant 25, 1147–1155 (2000). https://doi.org/10.1038/sj.bmt.1702420

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