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An accelerrated-phase CML patient with e19a2 junction of BCR/ABL gene - the first case of transplanted CML with micro bcr

Bone Marrow Transplantation volume 25, pages 1109–1110 (2000)Cite this article

Three breakpoint cluster regions (bcr) in the BCR gene have so far been reported in chronic myelogenous leukemia (CML) patients: the major bcr BCR/ABL gene encoding the P210 protein, which was found in the vast majority of patients; the minor bcr BCR/ABL gene (otherwise typical for Philadelphia-positive acute lymphoblastic leukemia) encoding the P190 protein; and the micro bcr gene encoding the P230 protein in a few cases of CML.1 The micro bcr gene was originally reported in six cases of CML with a mild course of the disease, which were classified as chronic neutrophilic leukemia (CNL).2345 One case of myeloid blast crisis of CML,6 another case of putative myeloid blast crisis of CML7 and two cases of ‘classical’ CML89 were also reported with this type of the BCR/ABL gene. We report here a CML patient with the micro bcr gene who went into an accelerated phase in which she was transplanted.

The RT-PCR of RNA from peripheral blood leukocytes with BCR e13 and ABL a3 primers,10 and the second nested PCR with BCR e19 and ABL a2 primers,2 performed according to a procedure described elsewhere,7 showed, before the first transplantation, a band of the same size as the previously reported e19a2 micro bcr patient7 (Figure 1a and b).

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Figure 1

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Acknowledgements

We thank Mrs R Maláčová for her skilled technical assistance and Mrs Š Haškovcová for her help with preparation of the figures. This work was supported by a grant No. 3563–3 of the Internal Grant Agency of the Ministry of Health Czech Republic.

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  1. Dept Molecular Genetics, Institute of Hematology and Blood Transfusion, U nemocnice 1, Prague, 2, 128 20, Czech Republic

    J Polák & C Haškovec

  2. Dept of Hemato-oncology, Faculty Hospital, Plzeň, Czech Republic

    V Koza & P Cetkovský

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  1. J Polák
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Polák, J., Koza, V., Cetkovský, P. et al. An accelerrated-phase CML patient with e19a2 junction of BCR/ABL gene - the first case of transplanted CML with micro bcr. Bone Marrow Transplant 25, 1109–1110 (2000). https://doi.org/10.1038/sj.bmt.1702412

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